Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
University of the Chinese Academy of Sciences, Beijing, 100049, China.
Protoplasma. 2023 Mar;260(2):483-495. doi: 10.1007/s00709-022-01790-x. Epub 2022 Jul 6.
These YABBY genes are transcription factors (TFs) that play crucial roles in various developmental processes in plants. There is no comprehensive characterization of YABBY genes in a valuable Chinese orchid herb, Dendrobium officinale. In this study, a total of nine YABBY genes were identified in the D. officinale genome. These YABBY genes were divided into four subfamilies: CRC/DL, FIL, INO, and YAB2. Expression pattern analyses showed that eight of the YABBY genes were strongly expressed in reproductive organs (flower buds) but weakly expressed in vegetative organs (roots, leaves, and stems). DoYAB1, DoYAB5, DoDL1, and DoDL3 were abundant in the small flower bud stage, while DoDL2 showed no changes throughout flower development. In addition, DoDL1-3 genes were strongly expressed in the column, tenfold more than in sepals, petals, and the lip. DoYAB1 from the FIL subfamily, DoYAB2 from the YAB2 subfamily, DoYAB3 from the INO subfamily, and DoDL2 and DoDL3 from the CRC/DL subfamily were selected for further analyses. Subcellular localization analysis showed that DoYAB1-3, DoDL2, and DoDL3 were localized in the nucleus. DoYAB2 and DoYAB3 interacted strongly with DoWOX2 and DoWOX4, while DoYAB1 showed a weak interaction with DoWOX4. These results reveal a regulatory network involving YABBY and WOX proteins in D. officinale. Our data provide clues to understanding the role of YABBY genes in the regulation of flower development in this orchid and shed additional light on the function of YABBY genes in plants.
这些 YABBY 基因是转录因子（TFs），在植物的各种发育过程中发挥着关键作用。在有价值的中国兰花植物铁皮石斛中，尚未对 YABBY 基因进行全面表征。在本研究中，从铁皮石斛基因组中鉴定出了总共 9 个 YABBY 基因。这些 YABBY 基因分为四个亚家族：CRC/DL、FIL、INO 和 YAB2。表达模式分析表明，8 个 YABBY 基因在生殖器官（花蕾）中强烈表达，而在营养器官（根、叶和茎）中弱表达。DoYAB1、DoYAB5、DoDL1 和 DoDL3 在小花蕾阶段丰富，而 DoDL2 在整个花发育过程中没有变化。此外，DoDL1-3 基因在柱中强烈表达，是萼片、花瓣和唇瓣的 10 倍。来自 FIL 亚家族的 DoYAB1、来自 YAB2 亚家族的 DoYAB2、来自 INO 亚家族的 DoYAB3 以及来自 CRC/DL 亚家族的 DoDL2 和 DoDL3 被选为进一步分析的对象。亚细胞定位分析表明，DoYAB1-3、DoDL2 和 DoDL3 定位于细胞核。DoYAB2 和 DoYAB3 与 DoWOX2 和 DoWOX4 强烈相互作用，而 DoYAB1 与 DoWOX4 表现出弱相互作用。这些结果揭示了一个涉及 YABBY 和 WOX 蛋白的调控网络在铁皮石斛中的作用。我们的数据为理解 YABBY 基因在兰花花发育调控中的作用提供了线索，并进一步阐明了 YABBY 基因在植物中的功能。
