Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2023 Jun 15;24(12):10165. doi: 10.3390/ijms241210165.
The small plant-specific YABBY gene family plays key roles in diverse developmental processes in plants. , , and are perennial herbaceous plants belonging to Orchidaceae with a high ornamental value. However, the relationships and specific functions of the YABBY genes in the species remain unknown. In this study, six s, nine s, and nine s were identified from the genome databases of the three species, which were unevenly distributed on five, eight, and nine chromosomes, respectively. The 24 YABBY genes were classified into four subfamilies (CRC/DL, INO, YAB2, and FIL/YAB3) based on their phylogenetic analysis. A sequence analysis showed that most of the YABBY proteins contained conserved C2C2 zinc-finger and YABBY domains, while a gene structure analysis revealed that 46% of the total YABBY genes contained seven exons and six introns. All the YABBY genes harbored a large number of Methyl Jasmonate responsive elements, as well as anaerobic induction -acting elements in the promoter regions. Through a collinearity analysis, one, two, and two segmental duplicated gene pairs were identified in the , , and genomes, respectively. The values of these five gene pairs were lower than 0.5, indicating that the YABBY genes underwent negative selection. In addition, an expression analysis revealed that plays a role in ovary and early-stage petal development, while is essential for lip development and is crucial for early sepal formation. primarily regulates sepals during blooming. Furthermore, there is the potential involvement of and in gynostemium development. The results of a comprehensive genome-wide study would provide significant clues for future functional investigations and pattern analyses of YABBY genes in different flower parts during flower development in the species.
小型植物特异性 YABBY 基因家族在植物的多种发育过程中发挥关键作用。 、 和 是兰科多年生草本植物，具有很高的观赏价值。然而，YABBY 基因在 物种中的关系和特定功能尚不清楚。在本研究中，从三个 物种的基因组数据库中鉴定出六个 、九个 和九个 ，它们分别不均匀地分布在五条、八条和九条染色体上。根据系统发育分析，将 24 个 YABBY 基因分为四个亚家族（CRC/DL、INO、YAB2 和 FIL/YAB3）。序列分析表明，大多数 YABBY 蛋白含有保守的 C2C2 锌指和 YABBY 结构域，而基因结构分析表明，总 YABBY 基因的 46%含有七个外显子和六个内含子。所有 YABBY 基因在启动子区域都含有大量的茉莉酸甲酯响应元件以及厌氧诱导作用元件。通过共线性分析，在 、 和 基因组中分别鉴定出一对、两对和两对片段重复基因对。这些五个基因对的 值均低于 0.5，表明 YABBY 基因经历了负选择。此外，表达分析表明， 在卵巢和早期花瓣发育中起作用，而 对于唇的发育是必需的， 对于早期萼片的形成至关重要。 主要在开花期间调节萼片。此外， 和 可能参与雄蕊的发育。这项全面的全基因组研究结果将为未来在花发育过程中不同花部 YABBY 基因的功能研究和模式分析提供重要线索。
