Sarkar Varun Kumar, De Ujjwal Kumar, Kala Anju, Verma Ashok Kumar, Chauhan Anuj, Paul Babul Rudra, Soni Srishti, Gandhar Jitendra Singh, Chaudhuri Pallab, Patra Manas Kumar, Eregowda Chethan Gollahalli, Gaur Gyanendra Kumar
Division of Medicine, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122 (UP), India.
Division of Animal Nutrition, ICAR-Indian Veterinary Research Institute, Izatnagar, 243122 (UP), India.
Probiotics Antimicrob Proteins. 2023 Feb;15(1):149-159. doi: 10.1007/s12602-022-09964-y. Epub 2022 Jul 6.
The aim of this study was to determine the effects of early-life bovine lactoferrin and host specific probiotic interventions on growth performance, mortality, and concentrations of immunoglobulin A and immunoglobulin G and transforming growth factor beta 1 (a marker of intestinal integrity) in serum of neonatal piglets. A total of eight piglet litters from parity matched sows were randomly divided into four groups and assigned to one of the four interventions: control (sterile normal saline), bovine lactoferrin (100 mg bovine lactoferrin), probiotic (1 × 10 colony forming unit (cfu) of swine origin Pediococcus acidilactici FT28 probiotic), and bovine lactoferrin + probiotic (100 mg bovine lactoferrin and 1 × 10 CFU of P. acidilactici FT28 probiotic). All the interventions were given once daily through oral route for first 7 days of life. The average daily gain (p = 0.0004) and weaning weight (p < 0.0001) were significantly improved in the probiotic group. The piglet survivability was significantly higher in bovine lactoferrin and probiotic groups than control group in Log-rank (Mantel-Cox) test. The concentrations of immunoglobulin A on day 21 in bovine lactoferrin, probiotic, and bovine lactoferrin + probiotic groups increased significantly (p < 0.05). Immunoglobulin G concentrations on day 7 and 15 in bovine lactoferrin and bovine lactoferrin + probiotic groups and on day 15 in probiotic group were significantly (p < 0.05) elevated, whereas, the concentration of transforming growth factor-β1 was significantly (p < 0.05) increased from day 7 to 21 in all the supplemented groups. In conclusion, the early-life bovine lactoferrin and P. acidilactici FT28 probiotic interventions reduced the mortality in the suckling piglets by promoting the systemic immunity and enhancing the intestinal integrity.
本研究的目的是确定早期牛乳铁蛋白和宿主特异性益生菌干预对新生仔猪生长性能、死亡率以及血清中免疫球蛋白A、免疫球蛋白G和转化生长因子β1（肠道完整性标志物）浓度的影响。从经产母猪中选取八窝仔猪，随机分为四组，分别接受四种干预措施之一：对照组（无菌生理盐水）、牛乳铁蛋白组（100毫克牛乳铁蛋白）、益生菌组（1×10个来源于猪的嗜酸乳杆菌FT28益生菌菌落形成单位（cfu））和牛乳铁蛋白+益生菌组（100毫克牛乳铁蛋白和1×10个cfu的嗜酸乳杆菌FT28益生菌）。在仔猪出生后的前7天，所有干预措施均通过口服途径每日给药一次。益生菌组的平均日增重（p = 0.0004）和断奶体重（p < 0.0001）显著提高。在对数秩（Mantel-Cox）检验中，牛乳铁蛋白组和益生菌组的仔猪存活率显著高于对照组。牛乳铁蛋白组、益生菌组和牛乳铁蛋白+益生菌组在第21天时免疫球蛋白A的浓度显著升高（p < 0.05）。牛乳铁蛋白组和牛乳铁蛋白+益生菌组在第7天和第15天以及益生菌组在第15天时免疫球蛋白G的浓度显著升高（p < 0.05），而在所有补充组中，从第7天到第21天转化生长因子-β1的浓度显著升高（p < 0.05）。总之，早期牛乳铁蛋白和嗜酸乳杆菌FT28益生菌干预通过促进全身免疫和增强肠道完整性降低了哺乳仔猪的死亡率。
