State Key Laboratory of Drug Research, Centre for the Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
J Med Chem. 2022 Aug 11;65(15):10638-10654. doi: 10.1021/acs.jmedchem.2c00848. Epub 2022 Jul 6.
The -methyladenosine (mA) demethylase FTO is overexpressed in acute myeloid leukemia (AML) cells and promotes leukemogenesis. We previously developed tricyclic benzoic acid FB23 as a highly potent FTO inhibitor in vitro. However, it showed a moderate antiproliferative effect on AML cells. In this work, we performed a structure-activity relationship study of tricyclic benzoic acids as FTO inhibitors. The analog exhibited excellent inhibitory effects on FTO similar to that of FB23 in vitro. In contrast to FB23, exerted a strong antiproliferative effect on AML cells. Like knock down, upregulated and expression and increased the protein abundance while it downregulated expression and decreased MYC protein abundance. These genes are key FTO targets in AML cells. Finally, treatment improved the survival rate of MONOMAC6-transplanted NSG mice. Collectively, our data suggest that targeting FTO with tricyclic benzoic acid inhibitors may be a potential strategy for treating AML.
