Hashimoto K, Ito K, Kumakiri M, Headington J
Arch Dermatol. 1987 May;123(5):633-7.
Long-term use of a nylon brush for back scratching by a 53-year-old white woman was associated with the development of typical macular amyloidosis. EKH4 monoclonal antikeratin antibody, which recognizes 50-kd neutral and acidic keratin species, labeled this amyloid. Confirmation of amyloid substance in the lesion included positive staining with Dylon and thioflavin T; immunohistochemical reactions with monoclonal and polyclonal antibodies against elastic fiber microfibrils (NKH1 and anti-P component), immunoglobulins (IgG, IgM, and IgA), and complement (C3); and electron microscopic identification of 6- to 10-nm straight filaments. Type IV collagen staining demonstrated a breakage and/or thickening of the dermoepidermal basement membrane above the amyloid deposition in the papillary dermis. Electron microscopic findings confirmed this phenomenon.
一名53岁白人女性长期使用尼龙刷挠背，这与典型黄斑淀粉样变的发生有关。识别50-kd中性和酸性角蛋白种类的EKH4单克隆抗角蛋白抗体标记了这种淀粉样蛋白。病变中淀粉样物质的确认包括用Dylon和硫黄素T染色呈阳性；与抗弹性纤维微原纤维（NKH1和抗P成分）、免疫球蛋白（IgG、IgM和IgA）及补体（C3）的单克隆和多克隆抗体进行免疫组化反应；以及电子显微镜鉴定出6至10纳米的直丝。IV型胶原染色显示乳头真皮淀粉样沉积上方的真皮表皮基底膜有断裂和/或增厚。电子显微镜检查结果证实了这一现象。
