Faculty of Science & Technology, Meijo University.
Laboratory of Pharmaceutical Analytical Chemistry, Gifu Pharmaceutical University.
J Oleo Sci. 2022 Aug 4;71(8):1097-1106. doi: 10.5650/jos.ess22077. Epub 2022 Jul 6.
Fucoxanthin, a characteristic carotenoid found in brown seaweeds, has been reported to exert beneficial biological activities, including antiobesity and anticancer activities Moreover, the Z-isomers of this compound potentially have greater bioavailability and biological activities than the naturally predominant all-E-isomer. Therefore, the consumption of Z-isomer-rich fucoxanthin through daily meals and dietary supplements may have beneficial effects. In this study, we aimed to investigate the effects of different extraction conditions on the Z-isomer ratio and recovery of fucoxanthin obtained from Undaria pinnatifida using supercritical CO (SC-CO), particularly focusing on the high-temperature conditions that enhance thermal Z-isomerization. High-temperature SC-CO extraction at ≥ 120°C was found to enhance the thermal isomerization of fucoxanthin. For example, when the extraction was performed at 40, 80, 120, and 160°C and 30 MPa for 30 min with a co-solvent (ethanol), the total Z-isomer ratios were 11.7, 11.5, 18.7, and 26.5%, respectively. Furthermore, the high-temperature extraction significantly improved fucoxanthin recovery under high-pressure (≥ 30 MPa) conditions in the presence of the co-solvent. For example, when fucoxanthin was extracted at 40, 80, 120, and 160°C under the same conditions as above, the recoveries were 17.5, 20.6, 30.7, and 29.5%, respectively. Hence, the high-temperature SC-CO extraction of fucoxanthin from U. pinnatifida would not only enhance health benefits of fucoxanthin via the Z-isomerization but also improve the productivity. Moreover, the use of non-toxic CO and a low-toxicity organic solvent (ethanol) ensures that the final fucoxanthin product is safe for consumption. The Z-isomer-rich fucoxanthin obtained using this method is accordingly considered to have potential for use as a dietary supplement.
岩藻黄质是一种存在于褐藻中的特征类胡萝卜素，已被报道具有有益的生物学活性，包括抗肥胖和抗癌活性。此外，该化合物的 Z-异构体比天然主要的全-E-异构体具有更高的生物利用度和生物学活性。因此，通过日常膳食和膳食补充剂摄入富含 Z-异构体的岩藻黄质可能具有有益的效果。在这项研究中，我们旨在研究不同提取条件对采用超临界 CO（SC-CO）从裙带菜中获得的岩藻黄质的 Z-异构体比例和回收率的影响，特别是关注可增强热 Z-异构体化的高温条件。发现高温 SC-CO 提取（≥120°C）可增强岩藻黄质的热异构化。例如，当在 40、80、120 和 160°C 和 30 MPa 下用共溶剂（乙醇）提取 30 分钟时，总 Z-异构体比例分别为 11.7、11.5、18.7 和 26.5%。此外，在共溶剂存在下，高温提取在高压（≥30 MPa）条件下显著提高了岩藻黄质的回收率。例如，当在上述相同条件下在 40、80、120 和 160°C 下提取岩藻黄质时，回收率分别为 17.5、20.6、30.7 和 29.5%。因此，从裙带菜中高温 SC-CO 提取岩藻黄质不仅可以通过 Z-异构体化增强岩藻黄质的健康益处，还可以提高生产力。此外，使用无毒的 CO 和低毒性的有机溶剂（乙醇）确保最终的岩藻黄质产品可安全食用。通过这种方法获得的富含 Z-异构体的岩藻黄质因此被认为具有作为膳食补充剂的潜力。
