Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, Ourense Campus, Universidade de Vigo, E-32004 Ourense, Spain.
REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr António Bernardino de Almeida 431, 4200-072 Porto, Portugal.
Mar Drugs. 2023 Jul 21;21(7):414. doi: 10.3390/md21070414.
Fucoxanthin (Fx) has been proven to exert numerous biological properties, which makes it an interesting molecule with diverse industrial applications. In this study, the kinetic behavior of Fx was studied to optimize three variables: time (-3 min to 7 days), temperature (-5 to 85 °C), and concentration of ethanol in water (-50 to 100%, /), in order to obtain the best Fx yield from using conventional heat extraction. The Fx content () was found through HPLC-DAD and expressed in µg Fx/g of algae sample dry weight (AS dw). Furthermore, extraction yield () was also found through dry weight analysis and was expressed in mg extract (E)/g AS dw. The purity of the extracts () was found and expressed in mg Fx/g E dw. The optimal conditions selected for were = 45 °C, = 70%, and = 66 min, obtaining ~5.24 mg Fx/g AS; for were = 65 °C, = 60%, and = ~10 min, obtaining ~450 mg E/g AS; and for were = 45 °C, = 70%, and = 45 min, obtaining ~12.3 mg Fx/g E. In addition, for the selected optimums, a full screening of pigments was performed by HPLC-DAD, while phenolics and flavonoids were quantified by spectrophotometric techniques and several biological properties were evaluated (namely, antioxidant, antimicrobial, and cholinesterase inhibitory activity). These results could be of interest for future applications in the food, cosmetic, or pharmaceutical industries, as they show the Fx kinetic behavior and could help reduce costs associated with energy and solvent consumption while maximizing the extraction yields.
岩藻黄质 (Fx) 已被证明具有多种生物学特性，这使其成为一种具有广泛工业应用前景的有趣分子。在本研究中，研究了 Fx 的动力学行为，以优化三个变量：时间（-3 分钟至 7 天）、温度（-5 至 85°C）和水乙醇浓度（-50 至 100%，/），以从使用常规热萃取的中获得最佳的 Fx 产量。通过 HPLC-DAD 测定 Fx 含量（），并以 µg Fx/g 藻样干重（AS dw）表示。此外，还通过干重分析测定提取产率（），并以 mg 提取物（E）/g AS dw 表示。通过测定提取物的纯度（），并以 mg Fx/g E dw 表示。选择的最佳条件为 = 45°C， = 70%， = 66 分钟，可获得约 5.24 mg Fx/g AS； = 65°C， = 60%， = 10 分钟，可获得约 450 mg E/g AS； = 45°C， = 70%， = 45 分钟，可获得约 12.3 mg Fx/g E。此外，对于选定的最佳条件，通过 HPLC-DAD 对色素进行了全面筛选，同时通过分光光度技术定量测定了酚类和类黄酮，并评估了几种生物学特性（即抗氧化、抗菌和胆碱酯酶抑制活性）。这些结果可能对食品、化妆品或制药行业的未来应用感兴趣，因为它们展示了 Fx 的动力学行为，并有助于降低与能源和溶剂消耗相关的成本，同时最大限度地提高提取产率。
