Department of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, No.16, 3rd Section, South Renmin Road, 610041, Chengdu, Sichuan, China.
R&D Center, Inner Mongolia Meng Niu Dairy Industry (Group) Co.,Ltd, 011500, Hohhot, Inner Mongolia, China.
Exp Anim. 2022 Nov 10;71(4):468-480. doi: 10.1538/expanim.21-0182. Epub 2022 Jul 6.
In this study, C57BL/6J male mice were fed normal chow (NC; control) or a high-fat diet (HFD) for 12 weeks, and HFD mice were supplemented with oral administration of Streptococcus thermophilus MN-ZLW-002 (HFD + MN002); n=20/group. Body weight, visceral fat, blood glucose, blood lipids and liver lipid deposition increased in the HFD group, and the composition of gut microbiota, cecum short-chain fatty acids and fecal bile acids (BAs) also changed. Oral-fed MN-002 increased the relative abundances of Ruminococcaceae, Lachnospiraceae and Streptococcaceae and improved blood glucose, liver cholesterol deposition, and serum IL-10, CCL-3 and the fecal BAs composition. In conclusion, the high-fat diet changed the composition of bile acids by shaping the gut microbiota into an obese type, leading to metabolic disturbances. Streptococcus thermophilus MN-ZLW-002 regulated gut microbiota by adjusting the composition of bile acids and improved the perturbation caused by high-fat diets. However, the effect of MN002 observed in animal experiments needs to be verified by long-term clinical trials.
在这项研究中，将 C57BL/6J 雄性小鼠分为正常进食组（NC；对照组）或高脂饮食组（HFD），12 周后，HFD 组给予鼠李糖乳杆菌 MN-ZLW-002 灌胃补充（HFD+MN002）；n=20/组。结果发现，HFD 组小鼠体重、内脏脂肪、血糖、血脂和肝脏脂质沉积增加，肠道微生物组成、盲肠短链脂肪酸和粪便胆汁酸（BAs）也发生变化。MN-002 灌胃增加了瘤胃球菌科、毛螺菌科和链球菌科的相对丰度，改善了血糖、肝脏胆固醇沉积和血清 IL-10、CCL-3 以及粪便 BAs 组成。综上所述，高脂饮食通过将肠道微生物群塑造成肥胖型，改变了胆汁酸的组成，导致代谢紊乱。鼠李糖乳杆菌 MN-ZLW-002 通过调节胆汁酸组成来调节肠道微生物群，改善了高脂饮食引起的紊乱。然而，动物实验中观察到的 MN002 效果需要通过长期临床试验来验证。
