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一个受体-通道三聚体为花粉管感受传导钙离子信号。

A receptor-channel trio conducts Ca signalling for pollen tube reception.

机构信息

Department of Plant and Microbial Biology, University of California at Berkeley, Berkeley, CA, USA.

College of Life Sciences, Capital Normal University, Beijing, China.

出版信息

Nature. 2022 Jul;607(7919):534-539. doi: 10.1038/s41586-022-04923-7. Epub 2022 Jul 6.

DOI:10.1038/s41586-022-04923-7
PMID:35794475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9308748/
Abstract

Precise signalling between pollen tubes and synergid cells in the ovule initiates fertilization in flowering plants. Contact of the pollen tube with the ovule triggers calcium spiking in the synergids that induces pollen tube rupture and sperm release. This process, termed pollen tube reception, entails the action of three synergid-expressed proteins in Arabidopsis: FERONIA (FER), a receptor-like kinase; LORELEI (LRE), a glycosylphosphatidylinositol-anchored protein; and NORTIA (NTA), a transmembrane protein of unknown function. Genetic analyses have placed these three proteins in the same pathway; however, it remains unknown how they work together to enable synergid-pollen tube communication. Here we identify two pollen-tube-derived small peptides that belong to the rapid alkalinization factor (RALF) family as ligands for the FER-LRE co-receptor, which in turn recruits NTA to the plasma membrane. NTA functions as a calmodulin-gated calcium channel required for calcium spiking in the synergid. We also reconstitute the biochemical pathway in which FER-LRE perceives pollen-tube-derived peptides to activate the NTA calcium channel and initiate calcium spiking, a second messenger for pollen tube reception. The FER-LRE-NTA trio therefore forms a previously unanticipated receptor-channel complex in the female cell to recognize male signals and trigger the fertilization process.

摘要

花粉管和胚珠中的助细胞之间精确的信号传递启动了开花植物的受精过程。花粉管与胚珠接触会引发助细胞中的钙爆发，从而诱导花粉管破裂和精子释放。这个过程被称为花粉管接收，需要拟南芥中三种由助细胞表达的蛋白质的作用：FERONIA（FER），一种受体样激酶；LORELEI（LRE），一种糖基磷脂酰肌醇锚定蛋白；和 NORTIA（NTA），一种功能未知的跨膜蛋白。遗传分析将这三种蛋白质置于同一途径中；然而，它们如何协同作用以实现助细胞-花粉管通讯仍然未知。在这里，我们鉴定了两个属于快速碱化因子（RALF）家族的花粉管衍生的小肽作为 FER-LRE 共受体的配体，而 FER-LRE 共受体反过来又将 NTA 募集到质膜上。NTA 作为钙调蛋白门控钙通道发挥作用，是助细胞中钙爆发所必需的。我们还重建了生化途径，其中 FER-LRE 感知花粉管衍生的肽以激活 NTA 钙通道并引发钙爆发，钙爆发是花粉管接收的第二信使。因此，FER-LRE-NTA 三联体在雌性细胞中形成了一个以前未预料到的受体-通道复合物，以识别雄性信号并触发受精过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c1e5/9308748/7cdb40d74e5e/nihms-1816205-f0004.jpg
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