Department of Stomatology, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes, 2227, São Paulo, SP, Brazil.
Division of Periodontology, Dental School, University of Taubaté, Rua dos Operários, 09, Centro, Taubaté, SP, Brazil.
Curr Stem Cell Res Ther. 2023;18(7):958-978. doi: 10.2174/1574888X17666220706092520.
Cell sheet technique using mesenchymal stem cells is a high-level strategy in periodontal regenerative medicine. Although recent studies have shown the role of MSCSs in increased dental supporting tissues and bone, there is no systematic review focused specifically on assessing periodontal regeneration in orthotopic animal models.
To evaluate the potential of mesenchymal stem cell sheets (MSCSs) on periodontal regeneration, compared to control, in experimental animal models Methods: Pre-clinical studies in periodontal defects of animal models were considered eligible. The electronic search included the MEDLINE, Web of Science, EMBASE and LILACS databases. The review was conducted according to the Preferred Reporting Item for Systematic Reviews and Meta-Analyses statement guidelines.
A total of 17 of the 3989 studies obtained from the electronic database search were included. MSCSs included dental follicle (DF) MSCSs, periodontal ligament (PL) MSCSs, dental pulp (DP) MSCSs, bone marrow (BM) MSCSs, alveolar periosteal (AP) MSCSs and gingival (G) MSCSs. Regarding cell sheet inducing protocol, most of the studies used ascorbic acid (52.94%). Others used culture dishes grafted with a temperature-responsive polymer (47.06%). Adverse effects were not identified in the majority of studies. Meta-analysis was not considered because of methodological heterogeneities. PDL-MSCSs were superior for periodontal regeneration enhancement compared to the control, but in an induced inflammatory microenvironment, DF-MSCSs were better. Moreover, DF-MSCSs, DP-MSCSs, and BM-MSCSs showed improved results compared to the control.
MSCSs can improve periodontal regeneration in animal periodontal defect models.
使用间充质干细胞的细胞片技术是牙周再生医学的高级策略。尽管最近的研究表明间充质干细胞在增加牙齿支持组织和骨方面的作用，但没有专门针对评估在原位动物模型中牙周再生的系统评价。
评估间充质干细胞片（MSCSs）在牙周缺损动物模型中相对于对照的牙周再生潜力。
符合条件的研究为牙周缺损动物模型的临床前研究。电子检索包括 MEDLINE、Web of Science、EMBASE 和 LILACS 数据库。综述按照系统评价和荟萃分析报告的首选项目进行。
从电子数据库搜索中获得的 3989 项研究中有 17 项被纳入。MSCSs 包括牙囊（DF）MSCSs、牙周韧带（PL）MSCSs、牙髓（DP）MSCSs、骨髓（BM）MSCSs、牙槽骨膜（AP）MSCSs 和牙龈（G）MSCSs。关于细胞片诱导方案，大多数研究使用抗坏血酸（52.94%）。其他研究使用与温度响应聚合物接枝的培养皿（47.06%）。大多数研究未发现不良反应。由于方法学的异质性，未考虑进行荟萃分析。与对照组相比，PDL-MSCSs 更有利于牙周再生增强，但在诱导的炎症微环境中，DF-MSCSs 更好。此外，DF-MSCSs、DP-MSCSs 和 BM-MSCSs 与对照组相比显示出更好的结果。
MSCSs 可以改善动物牙周缺损模型中的牙周再生。
