Department of Oral Implantology, Osaka Dental University, 1-5-17 Otemae Chuoku, Osaka, 540-0008, Japan.
Department of Infectious Diseases and Applied Immunology, IMSUT Hospital of The Institute of Medical Science, The University of Tokyo, 4-6-1 Shiroganedai, Minato-ku, Tokyo, 108-8639, Japan.
Stem Cell Res Ther. 2020 May 12;11(1):175. doi: 10.1186/s13287-020-01683-x.
Advances in regenerative medicine with stem cells have led to clinical trials. Dental/oral tissues are emerging as promising cellular sources of human mesenchymal stem cells. Recently, dental tissue-derived cells have been used clinically due to their great potential, easy accessibility, and ability to be obtained via methods with low invasiveness. The aim of this study is to systematically assess the clinical effectiveness of dental cell-mediated therapies compared to current evidence-based methods in human patients.
The electronic databases MEDLINE, Cochrane Central Register of Controlled Trials (CENTRAL), and ClinicalTrials.gov were searched up to December 2019 for clinical trials. Clinical trials with any intervention using stem cells/cells derived from dental tissue were included.
A total of 815 studies were identified by the electronic search, and 38 articles qualified for full-text evaluation. Finally, 20 studies (10 clinical trials using dental pulp-derived cells, 3 clinical trials using periodontal ligament-derived cells, and 7 studies using gingiva-derived cells) were included in this review. No clinical trials using dental follicle- or apical papilla-derived cells were selected in this review. Dental pulp-derived cells were used in clinical trials for bone regeneration, periodontitis, and dental pulp regeneration. All clinical trials using periodontal ligament-derived cells and gingiva-derived cells were conducted for periodontal disease treatment and gingival augmentation, respectively. Among the 20 selected studies, 16 showed clinical benefits of cell transplantation therapies. In addition, no study reported adverse events that may have been associated with cell transplantation.
These findings indicate that dental tissue-derived cells would be useful for cell-based regenerative medicine for various diseases.
再生医学领域的干细胞技术进步已经催生了临床试验。牙体/口腔组织正逐渐成为人类间充质干细胞有前途的细胞来源。由于其巨大的潜力、易于获取性以及可通过微创方法获得的能力，最近已在临床上使用了来源于牙组织的细胞。本研究旨在系统评估与当前基于证据的方法相比，牙科细胞介导疗法在人类患者中的临床效果。
截至 2019 年 12 月，我们通过电子数据库 MEDLINE、Cochrane 中央对照试验注册库（CENTRAL）和 ClinicalTrials.gov 对临床试验进行了检索。纳入了使用干细胞/源自牙齿组织的细胞进行任何干预的临床试验。
通过电子检索共确定了 815 项研究，有 38 篇文章符合全文评估标准。最终，本综述纳入了 20 项研究（10 项牙髓来源细胞的临床试验、3 项牙周膜来源细胞的临床试验和 7 项牙龈来源细胞的临床试验）。本综述未选择使用牙囊或根尖乳头来源细胞的临床试验。牙髓来源细胞已在骨再生、牙周炎和牙髓再生的临床试验中得到应用。所有使用牙周膜来源细胞和牙龈来源细胞的临床试验均分别用于牙周病治疗和牙龈增加。在这 20 项选定的研究中，有 16 项显示了细胞移植疗法的临床益处。此外，没有研究报告与细胞移植相关的不良事件。
这些发现表明，源自牙齿组织的细胞可能对各种疾病的基于细胞的再生医学有用。
