Escobar Jeffery, Dobbs Merilyn, Ellenberger Claudia, Parker Alysia, Latorre Juan D, Gabor Leslie
Elanco Animal Health, Greenfield, IN 46140, USA.
Elanco Australasia Pty. Ltd., Kemps Creek, NSW 2178, Australia.
Transl Anim Sci. 2022 Jun 15;6(3):txac079. doi: 10.1093/tas/txac079. eCollection 2022 Jul.
The importance of intestinal alkaline phosphatase (IAP) in maintaining gut health and intestinal homeostasis is well established. The objective of this study was to investigate the tolerance of poultry and swine to dietary supplementation of a novel microbial-derived alkaline phosphatase (AP; E.C. 3.1.3.1 produced by strain CMG3709). Studies were conducted on day-old Ross 308 chicken ( = 1,000; Study 1) and weaned piglets ( = 180; Study 2) for a duration of 42 d; and consisted of four treatment groups (TG) based on the concentration of microbial-derived AP supplemented in their diet at 0; 12,000; 20,000; and 200,000 U/kg of feed. Parameters such as animal survival, hematology, coagulation, and biochemical indices were assessed at the end of the study. The effect of microbial AP on nutrient absorption through skin pigmentation and intestinal permeability were also investigated in broilers ( = 600; Study 3). In poultry (Study 1), there were no statistically significant differences between control and TG for any of the hematological and biochemical parameters, except for a marginal increase ( < 0.05) in serum phosphorus at the highest dose. This variation was not dose-dependent, was well within the reference range, and was not associated with any clinical correlates. In swine (Study 2), hematological parameters such as leukocyte, basophil, and lymphocyte counts were lower ( < 0.05) for the two highest doses but were traced back to individual variations within the group. The biochemical indices in piglets showed no significant differences between control and supplemental groups except for glucose ( = 0.0005), which showed a high effect ( = 0.008) of the random blood collection order. Nonetheless, glucose was within the normal reference range, and were not related to in-feed supplementation of AP as they had no biological significance. The survival rate in all three studies was over 98%. Dietary supplementation of microbial-derived AP up to 16.7 times the intended use (12,000 U/kg feed) level had no negative effects in both poultry and swine. In-feed supplementation of microbial-derived AP for 28 d improved intestinal pigment absorption ( < 0.0001) and reduced intestinal paracellular permeability ( = 0.0001) in broilers (Study 3). Based on these results, it can be concluded that oral supplementation of microbial-derived AP is safe for poultry and swine and effective at improving gut health in poultry.
肠道碱性磷酸酶（IAP）在维持肠道健康和肠道内稳态方面的重要性已得到充分证实。本研究的目的是调查家禽和猪对日粮中添加一种新型微生物源碱性磷酸酶（AP；由CMG3709菌株产生的E.C. 3.1.3.1）的耐受性。对1日龄的罗斯308肉鸡（n = 1000；研究1）和断奶仔猪（n = 180；研究2）进行了为期42天的研究；根据日粮中添加的微生物源AP浓度分为四个处理组（TG），添加浓度分别为0；12,000；20,000；和200,000 U/kg饲料。在研究结束时评估动物存活率、血液学、凝血和生化指标等参数。还在肉鸡（n = 600；研究3）中研究了微生物AP对通过皮肤色素沉着和肠道通透性进行营养吸收的影响。在家禽中（研究1），除了最高剂量组血清磷有轻微升高（P < 0.05）外，对照组和各处理组之间的任何血液学和生化参数均无统计学显著差异。这种变化不依赖剂量，完全在参考范围内，且与任何临床相关因素无关。在猪中（研究2），两个最高剂量组的白细胞、嗜碱性粒细胞和淋巴细胞计数等血液学参数较低（P < 0.05），但可追溯到组内个体差异。仔猪的生化指标在对照组和添加组之间无显著差异，除了葡萄糖（P = 0.0005），随机采血顺序对其有高度影响（P = 0.008）。尽管如此，葡萄糖仍在正常参考范围内，且与日粮中添加AP无关，因为它们没有生物学意义。所有三项研究中的存活率均超过98%。日粮中添加高达预期使用水平（12,000 U/kg饲料）16.7倍的微生物源AP对家禽和猪均无负面影响。在肉鸡日粮中添加微生物源AP 28天可改善肠道色素吸收（P < 0.0001）并降低肠道细胞旁通透性（P = 0.0001）（研究3）。基于这些结果，可以得出结论，口服添加微生物源AP对家禽和猪是安全的，并且在改善家禽肠道健康方面是有效的。
