Mkhize Senzosenkosi Surprise, Cedric Simelane Mthokozisi Blessing, Mongalo Ishmael Nkoana, Pooe Ofentse Jacob
Discipline of Biochemistry, Westville Campus, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.
Department of Biochemistry, University of Johannesburg, P.O. Box 524, Auckland Park 2006, South Africa.
Biochem Res Int. 2022 Jun 27;2022:9436614. doi: 10.1155/2022/9436614. eCollection 2022.
mushroom contains important bioactive compounds and has several biological activities; however, mushroom growing substrates have major influence on chemical and functional characteristics of the mushroom. Hence, the study aimed to evaluate the influence of supplementing mushroom growing substrates with wheat bran (WB) towards yield/productivity, bioactive compounds, and antimicrobial and antioxidant activity of . . The mushroom was cultivated on sugarcane substrates supplemented with increasing levels of WB (0%-20%). The mushroom extracts were screened for bioactive compounds using gas chromatography-mass spectrometry (GC-MS). Antimicrobial activity was carried out using microplate assay, while antioxidant potential was investigated using reducing power assay. The addition of supplements on mushroom growing substrates had an influence on mushroom yield; hence, higher supplementation (18% and 20%) produced higher yield. The GC-MS revealed several bioactive compounds with known activity, such as vitamin E, phenol, fatty acids, and terpenoids. Concentration-dependent antioxidant activity was observed; hence, extracts at higher concentrations gave significantly higher reducing power. The extract had antimicrobial activity against all the tested organisms, with . showing high susceptibility to most of the extracts. However, mushrooms grown on bagasse substrates supplemented with 14% (0.02 mg/ml) and 20% WB (0.08 mg/ml) proved to have better antimicrobial activity on . The difference in susceptibility demonstrates that substrates type and composition could have an influence on bioactive compounds found within mushrooms, also influencing medicinal properties of edible mushroom. Thus, supplementing mushroom growing substrates not only improve yield, but also can contribute to bioactive compounds with medicinal potential.
蘑菇含有重要的生物活性化合物并具有多种生物活性；然而，蘑菇生长基质对蘑菇的化学和功能特性有重大影响。因此，本研究旨在评估用麦麸（WB）补充蘑菇生长基质对蘑菇产量/生产率、生物活性化合物以及抗菌和抗氧化活性的影响。在添加了不同水平WB（0%-20%）的甘蔗基质上栽培蘑菇。使用气相色谱-质谱联用仪（GC-MS）对蘑菇提取物进行生物活性化合物筛选。采用微孔板法进行抗菌活性检测，同时使用还原力法研究抗氧化潜力。在蘑菇生长基质上添加补充剂对蘑菇产量有影响；因此，较高的添加量（18%和20%）产生了更高的产量。GC-MS分析显示了几种具有已知活性的生物活性化合物，如维生素E、酚类、脂肪酸和萜类化合物。观察到浓度依赖性抗氧化活性；因此，较高浓度的提取物具有显著更高的还原力。该提取物对所有测试菌株均有抗菌活性，其中……对大多数提取物表现出高度敏感性。然而，在添加了14%（0.02mg/ml）和20%WB（0.08mg/ml）的蔗渣基质上生长的蘑菇对……表现出更好的抗菌活性。敏感性的差异表明基质类型和组成可能会影响蘑菇中发现的生物活性化合物，也会影响食用菌的药用特性。因此，补充蘑菇生长基质不仅可以提高产量，还可以产生具有药用潜力的生物活性化合物。
