Lampejo Arinola O, Hu Nien-Wen, Lucas Daniela, Lomel Banks M, Nguyen Christian M, Dominguez Carmen C, Ren Bing, Huang Yong, Murfee Walter L
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, United States.
Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL, United States.
Front Bioeng Biotechnol. 2022 Jun 20;10:912073. doi: 10.3389/fbioe.2022.912073. eCollection 2022.
The gap between and assays has inspired biomimetic model development. Tissue engineered models that attempt to mimic the complexity of microvascular networks have emerged as tools for investigating cell-cell and cell-environment interactions that may be not easily viewed . A key challenge in model development, however, is determining how to recreate the multi-cell/system functional complexity of a real network environment that integrates endothelial cells, smooth muscle cells, vascular pericytes, lymphatics, nerves, fluid flow, extracellular matrix, and inflammatory cells. The objective of this mini-review is to overview the recent evolution of popular biomimetic modeling approaches for investigating microvascular dynamics. A specific focus will highlight the engineering design requirements needed to match physiological function and the potential for top-down tissue culture methods that maintain complexity. Overall, examples of physiological validation, basic science discoveries, and therapeutic evaluation studies will emphasize the value of tissue culture models and biomimetic model development approaches that fill the gap between and assays and guide how vascular biologists and physiologists might think about the microcirculation.
[具体检测方法]与[具体检测方法]之间的差距推动了仿生模型的开发。试图模拟微血管网络复杂性的组织工程模型已成为研究细胞间和细胞与环境相互作用的工具，而这些相互作用可能难以直接观察到。然而，模型开发中的一个关键挑战是确定如何重现真实网络环境的多细胞/系统功能复杂性，该环境整合了内皮细胞、平滑肌细胞、血管周细胞、淋巴管、神经、流体流动、细胞外基质和炎症细胞。本综述的目的是概述用于研究微血管动力学的流行仿生建模方法的最新进展。一个特别的重点将突出匹配生理功能所需的工程设计要求以及维持复杂性的自上而下组织培养方法的潜力。总体而言，生理验证、基础科学发现和治疗评估研究的实例将强调组织培养模型和仿生模型开发方法的价值，这些方法填补了[具体检测方法]与[具体检测方法]之间的差距，并指导血管生物学家和生理学家如何思考微循环。
