Zhai Jiali, Ji Piyou, Xin Yu, Liu Yifan, Qu Qianwen, Han Wentong, Zhao Guangtao
School of Rehabilitation Medicine of Binzhou Medical University, Yantai, China.
Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.
Front Chem. 2022 Jun 20;10:899276. doi: 10.3389/fchem.2022.899276. eCollection 2022.
Rapid and highly sensitive detection of carcinoembryonic antigen (CEA) in blood could effectively improve the diagnostic sensitivity of colorectal cancer. In this work, a platinum microelectrode (PtμE) modified with gold nanoparticles was developed as a microsensor for the detection of CEA. As the recognition element, a CEA aptamer modified with sulfhydryl could be conjugated onto the surface of the PtμEs/Au. The quantitative analysis of the concentration of CEA [CEA] by the prepared PtμEs/Au aptasensor was carried out through square wave voltammetry. Under the optimized conditions, the PtμEs/Au aptasensor exhibits a linear response toward [CEA] in the range of 1.0 × 10-1.0 × 10 g/ml (S = 5.5 nA/dec, = 0.999), and the detection limit is 7.7 × 10 g/ml. The PtμEs/Au aptasensor also has good selectivity against other types of proteins existing in blood. The availability of the developed assay toward [CEA] in blood samples was investigated, and the results agreed well with those obtained through electrochemiluminescence provided by the hospital, and the volume of the blood sample for detection is only 20 μl. Herein, the proposed detection system could be used for the quantitative analysis of CEA in blood, with the advantages of high sensitivity, short time, and low cost. Moreover, the PtμEs/Au aptasensor has a potential application in clinical diagnosis.
快速且高度灵敏地检测血液中的癌胚抗原（CEA）可有效提高结直肠癌的诊断灵敏度。在这项工作中，开发了一种用金纳米颗粒修饰的铂微电极（PtμE）作为检测CEA的微传感器。作为识别元件，用巯基修饰的CEA适配体可缀合到PtμEs/Au的表面。通过方波伏安法对制备的PtμEs/Au适配体传感器进行CEA浓度[CEA]的定量分析。在优化条件下，PtμEs/Au适配体传感器对[CEA]在1.0×10 - 1.0×10 g/ml范围内呈现线性响应（S = 5.5 nA/dec， = 0.999），检测限为7.7×10 g/ml。PtμEs/Au适配体传感器对血液中存在的其他类型蛋白质也具有良好的选择性。研究了所开发的检测方法对血样中[CEA]的适用性，结果与医院通过电化学发光法获得的结果吻合良好，且检测所需血样体积仅为20μl。在此，所提出的检测系统可用于血液中CEA的定量分析，具有灵敏度高、时间短和成本低等优点。此外，PtμEs/Au适配体传感器在临床诊断中具有潜在应用。
