Zhai Jiali, Sun Huiyuan, Li Mingkang, Gao Yuhao, Hu Yixin, Gao Zhi, Xie Xiyu, Zhang Lixia, Zhao Guangtao
School of Rehabilitation Medicine of Binzhou Medical University Yantai 264003 China
Department of Critical Care Medicine, Yantai Yuhuangding Hospital Yantai 264003 China.
RSC Adv. 2023 Jul 17;13(31):21414-21420. doi: 10.1039/d3ra03759b. eCollection 2023 Jul 12.
The simple and sensitive detection of miRNA-122 in blood is crucially important for early hepatocellular carcinoma (HCC) diagnosis. In this work, a platinum microelectrode (PtμE) was prepared and electrodeposited with molybdenum disulfide (MoS) and gold nanoparticles (AuNP), respectively, and denoted as PtμE/MoS/Au. The prepared PtμE/MoS/Au was used as the microsensor for the detection of miRNA-122 combined with the probe DNA as a biorecognition element which is the complementary strand of miRNA-122. The PtμE/MoS/Au conjugated with the probe DNA modified with sulfydryl units was used as the micro-biosensor for the detection of miRNA-122. The square wave voltammetry was performed for the quantitative detection of miRNA-122 using [Fe(CN)] as a mediator. Under the optimized conditions, the PtμE/MoS/Au micro-biosensor shows a linear detection toward miRNA-122 ranging from 10 to 10 M ( = 6.9 nA dec, = 0.9997), and the detection limit is 1.6 × 10 M (3/). The PtμE/MoS/Au micro-biosensor demonstrates good selectivity against other types of proteins and small molecules, and has good reproducibility. Moreover, the PtμE/MoS/Au micro-biosensor was successfully applied for the measurement of miRNA-122 in real blood samples. Herein, the proposed detection assay could be a potential tool in HCC clinical diagnostics with high sensitivity.
血液中miRNA - 122的简单灵敏检测对于早期肝细胞癌（HCC）诊断至关重要。在本研究中，制备了铂微电极（PtμE），并分别用电沉积法负载二硫化钼（MoS）和金纳米颗粒（AuNP），记为PtμE/MoS/Au。将制备的PtμE/MoS/Au用作检测miRNA - 122的微传感器，结合作为生物识别元件的探针DNA，该探针DNA是miRNA - 122的互补链。用巯基修饰的与探针DNA共轭的PtμE/MoS/Au用作检测miRNA - 122的微生物传感器。使用[Fe(CN)]作为媒介物，通过方波伏安法对miRNA - 122进行定量检测。在优化条件下，PtμE/MoS/Au微生物传感器对miRNA - 122的检测呈线性，范围为10至10 M（ = 6.9 nA dec， = 0.9997），检测限为1.6×10 M（3/）。PtμE/MoS/Au微生物传感器对其他类型的蛋白质和小分子具有良好的选择性，且具有良好的重现性。此外，PtμE/MoS/Au微生物传感器已成功应用于实际血液样本中miRNA - 122的测量。在此，所提出的检测方法可能成为HCC临床诊断中具有高灵敏度的潜在工具。
