Department of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, United Kingdom.
FlexMedical Solutions, Eliburn Industrial Park, Livingston EH54 6GQ, United Kingdom.
Bioelectrochemistry. 2020 Jun;133:107480. doi: 10.1016/j.bioelechem.2020.107480. Epub 2020 Feb 3.
A flexible, thin-film carbon electrode is reported for detection of the key neurotransmitter dopamine using standard electroanalytical techniques of cyclic voltammetry, differential pulse voltammetry and square wave voltammetry. The thin-film electrode has been explored as a possible low-cost solution to detect low concentrations of dopamine and its performance has been compared with a commercially available screen printed carbon electrode. It was found that the thin-film electrode is more sensitive than the screen printed electrode, and can faithfully detect dopamine between 50 pM and 1 mM concentrations. The electrode provides a limit of detection of ~50 pM, displays good selectivity between dopamine and ascorbic acid, and is able to show a level of differentiation between the two compounds in terms of peak currents as well as oxidative potentials at physiologically relevant concentrations. This is in contrast to the screen printed electrode which is unable to discriminate between dopamine and ascorbic acid at the same concentrations. The key advantages of the presented electrode system are its low-cost, flexible substrate, and the ability to achieve very low levels of dopamine detection without requiring any electrode surface modification steps, a key factor in reducing fabrication costs and overall device complexity.
本文报道了一种灵活的薄膜碳电极，用于使用循环伏安法、差分脉冲伏安法和方波伏安法等标准电分析技术检测关键神经递质多巴胺。该薄膜电极作为一种可能的低成本解决方案，用于检测低浓度多巴胺，其性能与市售的丝网印刷碳电极进行了比较。结果表明，薄膜电极比丝网印刷电极更灵敏，能够在 50 pM 至 1 mM 浓度范围内准确检测多巴胺。该电极的检测限约为 50 pM，在多巴胺和抗坏血酸之间显示出良好的选择性，并且能够在生理相关浓度下，在峰电流和氧化电位方面显示出两种化合物之间的差异。相比之下，在相同浓度下，丝网印刷电极无法区分多巴胺和抗坏血酸。所提出的电极系统的主要优点是其低成本、灵活的基底，以及在无需任何电极表面修饰步骤的情况下实现非常低水平的多巴胺检测的能力，这是降低制造成本和整体器件复杂性的关键因素。
