Carmosino Ilaria, Bonardi Silvia, Rega Martina, Luppi Andrea, Lamperti Luca, Ossiprandi Maria Cristina, Bacci Cristina
Food Hygiene Unit, Department of Veterinary Science, University of Parma.
Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia-Romagna, Parma.
Ital J Food Saf. 2022 Jun 21;11(2):9972. doi: 10.4081/ijfs.2022.9972.
The European Food Safety Authority and European Centre of Disease Prevention and Control antimicrobial resistance report published in 2021 shows increasing levels of antimicrobial resistance in against antibiotics of choice for human salmonellosis (s-lactams and fluoroquinolones). The aim of the study was to follow the evolution of resistance against some Critical Important Antimicrobials in isolates from fresh pork collected in Emilia-Romagna region, northern Italy, over two decades. Emilia-Romagna region is characterized by production of well-known pork derived products, as Parma Ham. The samples were collected in three different periods, ranging from 2000 to 2003, 2012 to 2016 and 2018 to 2021. After serotyping, the isolates were phenotypically tested for resistance to three classes of antibiotics: s-lactams, fluoroquinolones and polymyxins. End-point polymerase chain reaction (PCR) and PCRReal Time were used for genotypical analyses. The phenotypical resistance to s-lactams and fluoroquinolones were clearly increasing when comparing the results obtained from isolates collected in the first period (16.7% and 16.7%, respectively) with those of the third period (29.7% and 32.4%, respectively). On the contrary, the resistance to colistin decreased from 33.3% to 5.4%. Genotypically, the 71.4% and 83.3% of the strains harboured s-lactams and fluoroquinolones genes, respectively, while colistin resistance genes were not detected in the phenotypically resistant strains.
欧洲食品安全局和欧洲疾病预防控制中心2021年发布的抗菌药物耐药性报告显示，针对人类沙门氏菌病的首选抗生素（β-内酰胺类和氟喹诺酮类），抗菌药物耐药性水平在上升。本研究的目的是追踪意大利北部艾米利亚-罗马涅地区20多年来从新鲜猪肉中分离出的菌株对一些关键重要抗菌药物的耐药性演变情况。艾米利亚-罗马涅地区以生产如帕尔马火腿等知名猪肉衍生产品而闻名。样本在三个不同时期采集，分别为2000年至2003年、2012年至2016年以及2018年至2021年。血清分型后，对分离株进行了针对三类抗生素的表型耐药性测试：β-内酰胺类、氟喹诺酮类和多粘菌素类。终点聚合酶链反应（PCR）和实时荧光定量PCR用于基因分型分析。将第一时期采集的分离株（分别为16.7%和16.7%）与第三时期的结果（分别为29.7%和32.4%）进行比较时，对β-内酰胺类和氟喹诺酮类的表型耐药性明显增加。相反，对粘菌素的耐药性从33.3%降至5.4%。在基因层面，分别有71.4%和83.3%的菌株携带β-内酰胺类和氟喹诺酮类基因，而在表型耐药菌株中未检测到粘菌素耐药基因。
