Rega Martina, Carmosino Ilaria, Bonilauri Paolo, Frascolla Viviana, Vismarra Alice, Bacci Cristina
Food Hygiene and Inspection Unit, Department of Veterinary Science, University of Parma, Strada del Taglio, 10, 43126 Parma, Italy.
Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna, 42124 Reggio Emilia, Italy.
Microorganisms. 2021 Jan 21;9(2):214. doi: 10.3390/microorganisms9020214.
A global increase in () resistant to cephalosporins (extended-spectrum β-lactamases (ESβLs) and AmpC β-lactamases) has been recorded in the last 20 years. Similarly, several studies have reported the spread of colistin resistance in Enterobacteriaceae isolated from food and the environment. The aim of the present study was to evaluate the prevalence of ESβL, AmpC and colistin-resistant isolated from pork and wild boar meat products in the Emilia Romagna region (North Italy). The isolates were analysed phenotypically (considering both resistant and intermediate profiles) and genotypically. The prevalence of genotypically confirmed ESβL and AmpC was higher in pork meat products (ESβL = 11.1% vs. AmpC = 0.3%) compared to wild boar meat (ESβL = 6.5% vs. AmpC = 0%). Intermediate profiles for cefotaxime (CTX) and ceftazidime (CAZ) were genotypically confirmed as ESβL in pork meat isolates but not for wild boar. Four from wild boar meat were resistant to colistin but did not harbour the gene. isolated from wild boar meat seem to show aspecific antimicrobial resistance mechanisms for cephalosporins and colistin. The prevalence of resistant isolates found in wild boar is less alarming than in pork from farmed domestic pigs. However, the potential risk to consumers of these meat products will require further investigations.
在过去20年中，已记录到对头孢菌素（超广谱β-内酰胺酶（ESβLs）和AmpCβ-内酰胺酶）耐药的情况在全球范围内有所增加。同样，几项研究报告了从食品和环境中分离出的肠杆菌科细菌中黏菌素耐药性的传播。本研究的目的是评估从意大利北部艾米利亚-罗马涅地区的猪肉和野猪肉制品中分离出的产ESβL、AmpC和耐黏菌素细菌的流行情况。对分离株进行了表型分析（同时考虑耐药和中介型）和基因型分析。与野猪肉（ESβL = 6.5%，AmpC = 0%）相比，猪肉制品中基因型确认的产ESβL和AmpC细菌的流行率更高（ESβL = 11.1%，AmpC = 0.3%）。猪肉分离株中头孢噻肟（CTX）和头孢他啶（CAZ）的中介型在基因型上被确认为ESβL，但野猪分离株中未被确认。从野猪肉中分离出的4株细菌对黏菌素耐药，但未携带该基因。从野猪肉中分离出的细菌似乎显示出对头孢菌素和黏菌素的非特异性抗菌耐药机制。在野猪中发现的耐药分离株的流行率不如养殖家猪的猪肉那样令人担忧。然而，这些肉制品对消费者的潜在风险需要进一步调查。
