Kappas A
Mutat Res. 1978 Aug;51(2):189-97. doi: 10.1016/s0027-5107(78)80018-9.
Four fungicides interfered with the segregation of chromosomes at mitosis of Aspergillus nidulans by increasing the somatic recombination, shown as colour sectors in green colonies, in a strain heterozygous for spore colour mutations. In an attempt to discover the mechanisms by which these fungicides increased the somatic recombination, a prototrophic diploid strain, heterozygous for colour and several other appropriate markers in all chromosomes, was used which enabled the detection and classification of all colour recombinants to be made by genetic analysis. The fungicides investigated were: benomyl (methyl-1-(butylcarbamoyl)-2-benzimidazole carbamate) a benzimidazole derivative, botran (2,6-dichloro-4-nitroaniline) and chloroneb (1,4-dichloro-2,5-dimethoxybenzene) of the aromatic hydrocarbon group of fungicides, and the antibiotic actinomycin D. At least three different mechanisms, non-disjunction, mitotic crossing-over and breakage-deletion, were found to be responsible for the recombinogenic activity of the compounds studied.
四种杀菌剂通过增加体细胞重组干扰了构巢曲霉有丝分裂时的染色体分离，在一个孢子颜色突变杂合的菌株中，体细胞重组表现为绿色菌落中的颜色扇形区域。为了探究这些杀菌剂增加体细胞重组的机制，使用了一个原养型二倍体菌株，它在所有染色体上对于颜色和其他几个合适的标记都是杂合的，这使得通过遗传分析能够对所有颜色重组体进行检测和分类。所研究的杀菌剂有：苯菌灵（甲基-1-（丁基氨基甲酰基）-2-苯并咪唑氨基甲酸酯），一种苯并咪唑衍生物；博特拉霉素（2,6-二氯-4-硝基苯胺）和克菌丹（1,4-二氯-2,5-二甲氧基苯），属于芳烃类杀菌剂；以及抗生素放线菌素D。至少发现了三种不同的机制，即不分离、有丝分裂交换和断裂-缺失，是所研究化合物重组活性的原因。
