Embrapa Soja, C.P. 231, 86001-970, Londrina, Paraná, Brazil.
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, SBN, Quadra 2, Bloco L, Lote 06, Edifício Capes, 70.040-020, Brasília, Distrito Federal, Brazil.
Int J Syst Evol Microbiol. 2022 Jul;72(7). doi: 10.1099/ijsem.0.005446.
is a heterogeneous bacterial genus capable of establishing symbiotic associations with a broad range of legume hosts, including species of economic and environmental importance. This study was focused on the taxonomic and symbiovar definition of four strains - CNPSo 4026, WSM 1704, WSM 1738 and WSM 4400 - previously isolated from nodules of legumes in Western Australia and South Africa. The 16S rRNA gene phylogenetic tree allocated the strains to the supergroup. The multilocus sequence analysis (MLSA) with partial sequences of six housekeeping genes , , , , and - did not cluster the strains under study as conspecific to any described species. Average nucleotide identity and digital DNA-DNA hybridization values were calculated for the four strains of this study and the closest species according to the MLSA phylogeny with the highest values being 95.46 and 62.20 %, respectively; therefore, both being lower than the species delineation cut-off values. The and phylogenies included strains WSM 1738 and WSM 4400 in the symbiovars retamae and vignae respectively, and also allowed the definition of three new symbiovars, sv. cenepequi, sv. glycinis, and sv. cajani. Analysis of morphophysiological characterization reinforced the identification of four novel proposed species that are accordingly named as follows: sp. nov. (CNPSo 4026=WSM 4798=LMG 31653), isolated from ; sp. nov. (WSM 1704=CNPSo 4028=LMG 31654), isolated from ; sp. nov. (WSM 1738=CNPSo 4025=LMG 31652), isolated from sp.; and sp. nov. (WSM 4400=CNPSo 4015=LMG 31648) isolated from sp.
是一个能够与广泛的豆科宿主建立共生关系的细菌属，包括具有经济和环境重要性的物种。本研究集中于从西澳大利亚和南非豆科植物根瘤中分离出的 4 株菌（CNPSo 4026、WSM 1704、WSM 1738 和 WSM 4400）的分类和共生型定义。16S rRNA 基因系统发育树将这些菌株分配到超级群中。利用 6 个看家基因（、、、、和）的部分序列进行的多位点序列分析（MLSA）并未将研究中的菌株聚类为任何描述的种内同物。根据 MLSA 系统发育树，对本研究的 4 株菌和最接近的种进行了平均核苷酸同一性和数字 DNA-DNA 杂交值的计算，最高值分别为 95.46%和 62.20%；因此，两者均低于种间划分的临界值。和系统发育树将菌株 WSM 1738 和 WSM 4400 分别归入共生型 retamae 和 vignae，并且还允许定义三个新的共生型，sv. cenepequi、sv. glycinis 和 sv. cajani。形态生理学特征分析加强了对 4 种新提出的种的鉴定，它们分别被命名为：sp. nov.（CNPSo 4026=WSM 4798=LMG 31653），分离自；sp. nov.（WSM 1704=CNPSo 4028=LMG 31654），分离自；sp. nov.（WSM 1738=CNPSo 4025=LMG 31652），分离自；和 sp. nov.（WSM 4400=CNPSo 4015=LMG 31648），分离自。
