环状 RNA 作为系统性红斑狼疮生物标志物的意义：系统评价和荟萃分析。
Significance of circRNAs as biomarkers for systemic lupus erythematosus: a systematic review and meta-analysis.
机构信息
Department of Dermatology and Venerology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Department of Dermatology, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi, China.
出版信息
J Int Med Res. 2022 Jul;50(7):3000605221103546. doi: 10.1177/03000605221103546.
OBJECTIVE
To comprehensively evaluate the significance of circular RNAs (circRNAs) as potential diagnostic biomarkers for systemic lupus erythematosus (SLE) via pooled analyses of data from published studies that focussed on the association between circRNAs and SLE.
METHODS
The systematic review and meta-analysis protocol was registered in PROSPERO (registration No. CRD42021229383). Relevant studies published before 3 April 2022 were selected to verify the relationship between circRNA expression levels and SLE. Extracted data were analysed using a random-effects model with Meta-DiSc 1.4 and Stata 16 software. Transcription factors related to hsa_circ_0000479 and its parental gene were extracted from the TRCirc and hTFtarget databases, respectively.
RESULTS
A total of 10 studies, involving 438 patients with SLE and 434 controls, were included in the meta-analysis. The pooled sensitivity, specificity, and diagnostic odds ratio of circRNAs in detecting SLE were 0.66 (95% confidence interval [CI] 0.63, 0.70), 0.79 (95% CI 0.76, 0.82), and 10.80 (95% CI 6.58, 17.73), respectively. The area under the summary receiver operating characteristic curve was 0.8366.
CONCLUSIONS
Meta-analysis of pooled data indicated a moderate accuracy of circRNAs in diagnosing SLE. The exact diagnostic value of circRNAs and the mechanisms of interaction between circRNAs and their parental genes should be confirmed in further studies.
目的
通过对集中探讨 circRNA 与系统性红斑狼疮（SLE）之间关联的已发表研究数据进行汇总分析，全面评估 circRNA 作为 SLE 潜在诊断生物标志物的意义。
方法
本系统评价和荟萃分析方案已在 PROSPERO（注册号：CRD42021229383）中注册。选择截至 2022 年 4 月 3 日之前发表的相关研究，以验证 circRNA 表达水平与 SLE 之间的关系。使用 Meta-DiSc 1.4 和 Stata 16 软件，采用随机效应模型对提取的数据进行分析。分别从 TRCirc 和 hTFtarget 数据库中提取与 hsa_circ_0000479 及其亲本基因相关的转录因子。
结果
共有 10 项研究，涉及 438 例 SLE 患者和 434 名对照者，被纳入荟萃分析。circRNAs 用于诊断 SLE 的汇总敏感性、特异性和诊断优势比分别为 0.66（95%置信区间 0.63，0.70）、0.79（95%置信区间 0.76，0.82）和 10.80（95%置信区间 6.58，17.73）。汇总受试者工作特征曲线下面积为 0.8366。
结论
汇总数据的荟萃分析表明，circRNAs 诊断 SLE 的准确性中等。circRNAs 的确切诊断价值以及 circRNAs 与其亲本基因之间相互作用的机制，应在进一步的研究中得到证实。
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