School of Materials and Chemistry, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, China.
Department of Critical Care Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, 200092, China.
Biomater Sci. 2022 Aug 9;10(16):4627-4634. doi: 10.1039/d2bm00538g.
Based on the necessity and urgency of cardiac troponin I (cTnI) detection for the diagnosis of myocardial infarction, a novel unlabeled photoelectrochemical (PEC) immunosensor has been developed to detect cTnI rapidly and sensitively. Silicon nanowire arrays (SiNWs) were prepared metal-assisted chemical etching. To improve the stability and sensitivity towards cTnI sensing, the surface of silicon nanowire arrays were coated with polydopamine by an growth method. PDA was uniformly modified on the nanowire surface to provide a reliable active site for antibody binding. The linear dynamic range of the cTnI detection method was 0.005-10 ng mL, and the detection limit was 1.47 pg mL. The designed PEC immunosensor exhibited good sensitivity, selectivity, stability and reproducibility. The electrode enabled label-free detection and provided a new route to realize point-of-care testing of cTnI.
基于心肌肌钙蛋白 I (cTnI) 检测在诊断心肌梗死中的必要性和紧迫性，开发了一种新型的无标记光电化学 (PEC) 免疫传感器，用于快速灵敏地检测 cTnI。采用金属辅助化学刻蚀法制备了硅纳米线阵列 (SiNWs)。为了提高对 cTnI 传感的稳定性和灵敏度，通过自 生长方法在硅纳米线阵列表面涂覆了聚多巴胺 (PDA)。PDA 均匀地修饰在纳米线表面，为抗体结合提供了可靠的活性位点。cTnI 检测方法的线性动态范围为 0.005-10ngmL，检测限为 1.47pgmL。设计的 PEC 免疫传感器表现出良好的灵敏度、选择性、稳定性和重现性。该电极实现了无标记检测，为实现 cTnI 的即时检测提供了新途径。
