USDA-ARS National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA.
Agdia Inc., Elkhart, IN, 46514, USA.
Arch Virol. 2022 Oct;167(10):2089-2092. doi: 10.1007/s00705-022-05520-9. Epub 2022 Jul 7.
Leaves from the ornamental plant Chaenostoma cordatum (Thunb.) Benth. expressing virus-like symptoms were collected for pathogen testing. A virus with features consistent with those of members of the genus Potexvirus was identified by high-throughput sequencing. The genome sequence was confirmed and completed using RT-PCR, cloning, rapid amplification of cDNA ends kits, and Sanger sequencing, revealing a complete viral genome of 6,071 nucleotides, excluding the poly-A tail. Phylogenetic analysis of the RNA-dependent RNA polymerase sequence from the viral genome indicated that its closest relative is Plantago asiatica mosaic virus. Further analysis of the nucleotide and amino acid sequences revealed that it had diverged enough from other potexviruses to be considered a member of a new species.
从表现病毒样症状的观赏植物Chaenostoma cordatum（Thunb.）Benth.中采集叶片进行病原体检测。通过高通量测序鉴定出一种与 Potexvirus 属成员特征一致的病毒。通过 RT-PCR、克隆、快速 cDNA 末端扩增试剂盒和 Sanger 测序对基因组序列进行了确认和完成，揭示了一个完整的病毒基因组，长度为 6071 个核苷酸，不包括 poly-A 尾。对病毒基因组 RNA 依赖性 RNA 聚合酶序列的系统进化分析表明，其最亲近的亲缘关系是车前草花叶病毒。对核苷酸和氨基酸序列的进一步分析表明，它与其他 Potexviruses 已经足够分化，可以被认为是一个新种的成员。
