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从肥胖天宝花中分离到的一种新马铃薯 Y 病毒的生物学和分子特征。

Biological and molecular characterization of a new potexvirus isolated from Adenium obesum.

机构信息

Universidade de São Paulo/Esalq, Depto. de Fitopatologia e Nematologia, Av. Pádua Dias 11, Piracicaba, 13418-900, Brazil.

Depto. de Biologia Molecular, Universidade de Brasília, Brasília , Federal District, Brazil.

出版信息

Arch Virol. 2023 Oct 14;168(11):272. doi: 10.1007/s00705-023-05889-1.

DOI:10.1007/s00705-023-05889-1
PMID:37837465
Abstract

Adenium obesum plants showing virus-like symptoms were collected in several regions of Brazil. Mottling symptoms like those observed in symptomatic plants in the field were reproduced in mechanically inoculated A. obesum plants. This potexvirus was named "desert rose mottle virus" (DRMoV), and its genome sequence was first determined by high-throughput sequencing and then confirmed by Sanger sequencing. The complete genome of DRMoV is 6,781 nt in length, excluding the poly(A) tail, and five ORFs were predicted in order from 5' to 3': Rep-TGB1-TGB2-TGB3-CP. Phylogenetic analysis based on Rep amino acid sequences showed different clustering among potexviruses. These data suggest that RDMoV is a new member of the genus Potexvirus, and the binomial name "Potexvirus adenii" is proposed for its species.

摘要

在巴西的几个地区采集到了表现病毒样症状的沙漠榕植株。在机械接种的沙漠榕植株中重现了田间有症状植株中观察到的斑驳症状。这种马铃薯 Y 病毒被命名为“沙漠玫瑰斑驳病毒”(DRMoV),其基因组序列首先通过高通量测序确定,然后通过 Sanger 测序确认。DRMoV 的完整基因组长度为 6781 个核苷酸,不包括 poly(A)尾巴,从 5'到 3'预测了五个 ORF:Rep-TGB1-TGB2-TGB3-CP。基于 Rep 氨基酸序列的系统发育分析显示马铃薯 Y 病毒属内存在不同的聚类。这些数据表明,RDMoV 是马铃薯 Y 病毒属的一个新成员,建议为其种名采用“Potexvirus adenii”。

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