Institute of Human Genetics (IGH), CNRS and University of Montpellier, Montpellier, France.
Methods Mol Biol. 2022;2509:53-68. doi: 10.1007/978-1-0716-2380-0_3.
Piwi-bound small RNAs induce programmed DNA elimination in the ciliated protozoan Tetrahymena. Using the phenomenon called codeletion, this process can be reprogrammed to induce ectopic DNA elimination at basically any given genomic location. Here, we describe the usage of codeletion for genetic studies in Tetrahymena and for investigations of the molecular mechanism of Piwi-directed programmed DNA elimination.
Piwi 结合的小 RNA 诱导纤毛原生动物四膜虫中的程序性 DNA 消除。利用称为共缺失的现象，这个过程可以被重新编程以在基本上任何给定的基因组位置诱导异位 DNA 消除。在这里，我们描述了共缺失在四膜虫中的遗传研究以及 Piwi 指导的程序性 DNA 消除的分子机制研究中的应用。
