Michigan State University College of Osteopathic Medicine Hill Orthopedics, US.
Institute for Quantitative Health Science and Engineering Michigan State University, US.
J Wound Care. 2022 Jul 1;31(Sup7):S41-S50. doi: 10.12968/jowc.2022.31.Sup7.S41.
The purpose of this study was to investigate the antimicrobial efficacy of a novel activated zinc solution against meticillin-resistant (MRSA) and after one hour, and to evaluate any untoward effect of the solution on local wound tissue at 24 hours after solution exposure in a pig wound model.
A pathogen-free, commercially raised, Yorkshire-cross female pig was acquired 12 days prior to the procedure. Within one week prior to the procedure, a small loopful of test bacteria, (pig-isolate) and MRSA (ATCC-6538), were streaked and cultured on a non-selective agar. Full-thickness wounds (n=24) were created and evenly divided into three groups: control wounds (exposed to bacteria but untreated, n=8); wounds treated with Compound 1 (n=8), and wounds treated with Compound 2 (n=8). All wounds were dressed and monitored for one hour and 24 hours.
After one hour, the wounds treated with Compound 1 and Compound 2 had a mean recoverable total bacteria of 2.8 log colony forming units (CFUs) and 3.5 logCFUs, respectively. After one hour, the wounds treated with Compound 1 and Compound 2 had a mean recoverable MRSA of 2.3 logCFUs and 1.6 logCFUs, respectively (p=0.009). After one hour, the wounds treated with Compound 1 and Compound 2 had a mean recoverable of 0.3 logCFUs and 0.0 logCFUs, respectively (p=0.000). After 24 hours of exposure to Compound 1 and Compound 2, there was no statistically significant increased necrosis (p=0.12, p=0.31, respectively) or neutrophilic infiltrate (Compound 2, p=0.12) when compared with control wounds.
The novel activated-zinc compound used in this study demonstrated a 99.5-99.9% reduction in total bacteria, a 99.9-99.98% reduction in MRSA, and 100% eradication of one hour after exposure. This novel solution may provide another significant tool to treat and/or prevent wound infections.
本研究旨在探究一种新型激活锌溶液对耐甲氧西林金黄色葡萄球菌（MRSA）和 的抗菌功效，并在猪创伤模型中观察该溶液在暴露 24 小时后对局部伤口组织的任何不良影响。
在手术前 12 天获得一头无病原体、商业化饲养的约克夏杂交雌性猪。在手术前一周内，用一小环测试细菌，（猪分离株）和 MRSA（ATCC-6538），并在非选择性琼脂上划线和培养。共创建 24 个全层伤口（n=24），并平均分为三组：对照组（暴露于细菌但未处理，n=8）；用 Compound 1 处理的伤口（n=8）和用 Compound 2 处理的伤口（n=8）。所有伤口均进行包扎并在 1 小时和 24 小时时进行监测。
1 小时后，用 Compound 1 和 Compound 2 处理的伤口的可回收总细菌数分别为 2.8 对数集落形成单位（CFU）和 3.5 对数 CFU。1 小时后，用 Compound 1 和 Compound 2 处理的伤口的可回收 MRSA 数分别为 2.3 对数 CFU 和 1.6 对数 CFU（p=0.009）。1 小时后，用 Compound 1 和 Compound 2 处理的伤口的可回收 数分别为 0.3 对数 CFU 和 0.0 对数 CFU（p=0.000）。暴露于 Compound 1 和 Compound 2 24 小时后，与对照组伤口相比，没有统计学上显著增加的坏死（p=0.12，p=0.31，分别）或中性粒细胞浸润（Compound 2，p=0.12）。
本研究中使用的新型激活锌化合物在暴露 1 小时后，总细菌减少了 99.5-99.9%，MRSA 减少了 99.9-99.98%， 完全消除。这种新型溶液可能为治疗和/或预防伤口感染提供另一种重要工具。
