Biomanufacturing Research Institute and Technology Enterprise (BRITE) and Department of Pharmaceutical Sciences, North Carolina Central University, Durham, North Carolina 27707, United States.
Department of Chemistry, Clemson University, Clemson, South Carolina 29634, United States.
ACS Appl Bio Mater. 2022 Jul 18;5(7):3158-3166. doi: 10.1021/acsabm.2c00153. Epub 2022 Jul 7.
The prevention of viral transmission is an important step to address the spread of viral infections. Using the enveloped vesicular stomatitis virus (VSV) as a model, this study explored the antiviral functions of the specifically designed and prepared carbon dots (CDots). The CDots were prepared using small carbon nanoparticles with surface functionalization-passivation by oligomeric polyethylenimine (PEI). The results indicated that the PEI-CDots were readily activated by visible light to effectively and efficiently inactivate VSVs under various combinations of experimental conditions (viral titer, dot concentration, and treatment time). The photodynamically induced viral structural protein degradation and genomic RNA degradation were observed, suggesting the mechanistic origins, leading to the inactivation of virus. The results suggested CDots as a class of promising broad-spectrum antiviral agents for disinfection of viruses.
病毒传播的预防是控制病毒感染传播的重要步骤。本研究以包膜的水疱性口炎病毒（VSV）为模型，探索了专门设计和制备的碳点（CDots）的抗病毒功能。通过寡聚聚亚乙基亚胺（PEI）对表面功能化-钝化的小碳纳米颗粒制备 CDots。结果表明，PEI-CDots 很容易被可见光激活，在各种实验条件（病毒滴度、点浓度和处理时间）下可有效且高效地灭活 VSV。观察到光动力诱导的病毒结构蛋白降解和基因组 RNA 降解，提示可能的作用机制，从而导致病毒失活。结果表明 CDots 作为一类有前途的广谱抗病毒剂，可用于病毒的消毒。
