利用低温电子断层扫描和相关显微镜技术对神经元突触进行数字化。

Digitalizing neuronal synapses with cryo-electron tomography and correlative microscopy.

机构信息

California NanoSystems Institute, University of California, Los Angeles, CA, USA; Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA, USA. Electronic address: https://twitter.com/yuntaoinnit.

Interdisciplinary Center for Brain Information, Brain Cognition and Brain Disease Institute, Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Faculty of Life and Health Sciences, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.

出版信息

Curr Opin Neurobiol. 2022 Oct;76:102595. doi: 10.1016/j.conb.2022.102595. Epub 2022 Jul 4.

DOI:10.1016/j.conb.2022.102595

PMID:35797809

Abstract

Synapses are the structural and functional joints of neuronal circuits, and brain function is fundamentally based on synaptic quantal transmission and plasticity. Precise mapping of key components within individual synapses in different states can reveal the principles governing synapse formation, transmission, and plasticity and improving understanding of the mechanisms of synapse-related diseases. Cryo-electron tomography (cryo-ET) and correlative microscopy are increasingly powerful tools that can dissect the molecular sociology of intact cells, including neuronal synapses. In this study, we discuss current progress made in cryo-ET studies assessing neuronal synapses, especially sample preparation, molecule identification, and correlative approaches for synaptic dynamics and functions.

摘要

突触是神经元回路的结构和功能连接，大脑功能从根本上基于突触量子传递和可塑性。在不同状态下，对单个突触内的关键组件进行精确映射，可以揭示支配突触形成、传递和可塑性的原理，从而增进对与突触相关疾病机制的理解。低温电子断层扫描（cryo-ET）和相关显微镜技术是越来越强大的工具，可以剖析包括神经元突触在内的完整细胞的分子社会学。在本研究中，我们讨论了评估神经元突触的 cryo-ET 研究中取得的当前进展，特别是针对突触动力学和功能的样本制备、分子鉴定和相关方法。