School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China; Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China.
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China; Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang 621010, China.
Carbohydr Polym. 2022 Oct 1;293:119696. doi: 10.1016/j.carbpol.2022.119696. Epub 2022 Jun 7.
Uncontrolled bleeding and infection following trauma remain a clinical challenge. Here, we developed a synergistic hemostatic and antibacterial aerogel dressing based on oxidized Bletilla rhizome polysaccharide Schiff Base (ORBPS) and polyvinyl alcohol (PVA) by using a combined freeze-drying/cross-linking process. Bletilla rhizome polysaccharide (RBP) was extracted by water extraction and alcohol precipitation. RBP contained mannose and glucose in the molar ratio of 2:1 and its molecular weight was 8.2 × 10 g/mol. ORBPS was prepared via Schiff base reaction between silver sulfadiazine and oxidized RBP (ORBP), and verified by FTIR and NMR spectra. The resultant ORBPS/PVA aerogel exhibited excellent antibacterial and hemostatic capabilities. The aerogel also showed good liquid absorption capacity and biocompatibility. Full-thickness skin defect experiments indicated the aerogel enhanced wound healing by reducing inflammation, promoting angiogenesis, and accelerating epithelialization. Therefore, ORBPS/PVA aerogel may be a potential hemostasis and anti-infection wound dressing.
创伤后无法控制的出血和感染仍然是临床面临的挑战。在这里，我们通过冷冻干燥/交联过程，开发了一种基于氧化白芨多糖席夫碱（ORBPS）和聚乙烯醇（PVA）的协同止血和抗菌气凝胶敷料。白芨多糖（RBP）通过水提醇沉法提取得到，RBP 中甘露糖和葡萄糖的摩尔比为 2:1，分子量为 8.2×10 g/mol。通过银磺胺嘧啶和氧化 RBP（ORBP）之间的席夫碱反应制备 ORBPS，并通过 FTIR 和 NMR 光谱进行验证。所得的 ORBPS/PVA 气凝胶表现出优异的抗菌和止血性能。气凝胶还表现出良好的液体吸收能力和生物相容性。全厚皮肤缺损实验表明，气凝胶通过减少炎症、促进血管生成和加速上皮化来促进伤口愈合。因此，ORBPS/PVA 气凝胶可能是一种有潜力的止血和抗感染伤口敷料。
