成人脑解剖用于膜片钳神经元。

Dissection of Adult Brains for Patch-Clamping Neurons.

机构信息

Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET), Partner Institute of the Max Planck Society, Buenos Aires C1425FQD, Argentina.

Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA-CONICET), Partner Institute of the Max Planck Society, Buenos Aires C1425FQD, Argentina

出版信息

Cold Spring Harb Protoc. 2022 Aug 1;2022(8):Pdb.prot107935. doi: 10.1101/pdb.prot107935.

DOI:10.1101/pdb.prot107935

PMID:35798465

Abstract

The brain of adult flies () has been studied in detail from several perspectives, including the anatomical and molecular characterization of hundreds of neuronal types. However, information regarding the electrophysiological properties of most neuronal types is lacking. This protocol provides detailed information on how to dissect the brain of adult flies to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as fly brains, to perform successful patch-clamp recordings is a critical step; here, we explain how this can be achieved using cyanoacrylate glue.

摘要

成年果蝇的大脑已经从多个角度进行了详细研究，包括数百种神经元类型的解剖学和分子特征分析。然而，大多数神经元类型的电生理特性信息还很缺乏。本方案提供了详细信息，介绍如何解剖成年果蝇的大脑，以制备可用于膜片钳记录的离体脑片。固定新鲜而微小的组织，如果蝇大脑，是进行成功膜片钳记录的关键步骤；在这里，我们解释如何使用氰基丙烯酸酯胶来实现这一点。