National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, P.R. China.
University of Chinese Academy of Sciences, Beijing, P.R. China.
Nat Plants. 2022 Jul;8(7):840-855. doi: 10.1038/s41477-022-01177-z. Epub 2022 Jul 7.
Non-photochemical quenching (NPQ) plays an important role for phototrophs in decreasing photo-oxidative damage. qH is a sustained form of NPQ and depends on the plastid lipocalin (LCNP). A thylakoid membrane-anchored protein SUPPRESSOR OF QUENCHING1 (SOQ1) prevents qH formation by inhibiting LCNP. SOQ1 suppresses qH with its lumen-located thioredoxin (Trx)-like and NHL domains. Here we report structural data, genetic modification and biochemical characterization of Arabidopsis SOQ1 lumenal domains. Our results show that the Trx-like and NHL domains are associated together, with the cysteine motif located at their interface. Residue E859, required for SOQ1 function, is pivotal for maintaining the Trx-NHL association. Importantly, the C-terminal region of SOQ1 forms an independent β-stranded domain that has structural homology to the N-terminal domain of bacterial disulfide bond protein D and is essential for negative regulation of qH. Furthermore, SOQ1 is susceptible to cleavage at the loops connecting the neighbouring lumenal domains both in vitro and in vivo, which could be a regulatory process for its suppression function of qH.
非光化学猝灭（NPQ）在减少光氧化损伤方面对光养生物起着重要作用。qH 是 NPQ 的持续形式，依赖于质体脂联素（LCNP）。类囊体膜锚定蛋白 SUPPRESSOR OF QUENCHING1（SOQ1）通过抑制 LCNP 来阻止 qH 的形成。SOQ1 通过其腔室定位的硫氧还蛋白（Trx）样和 NHL 结构域来抑制 qH。在这里，我们报告了拟南芥 SOQ1 腔室结构域的结构数据、遗传修饰和生化特性。我们的结果表明，Trx 样和 NHL 结构域彼此相关，半胱氨酸基序位于它们的界面上。对于 SOQ1 功能至关重要的残基 E859 对于维持 Trx-NHL 关联至关重要。重要的是，SOQ1 的 C 末端区域形成一个独立的β-折叠结构域，与细菌二硫键蛋白 D 的 N 末端结构域具有结构同源性，对于 qH 的负调控至关重要。此外，SOQ1 在体外和体内都容易在连接相邻腔室结构域的环处发生切割，这可能是其对 qH 抑制功能的调节过程。
