Schunck W H, Kärgel E, Gross B, Wiedmann B, Mauersberger S, Köpke K, Kiessling U, Strauss M, Gaestel M, Müller H G
Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch.
Biochem Biophys Res Commun. 1989 Jun 15;161(2):843-50. doi: 10.1016/0006-291x(89)92677-6.
A cDNA library was established starting from poly(A) RNA of n-alkane-grown Candida maltosa cells and cDNA clones were isolated containing the entire coding sequence for the alkane hydroxylating cytochrome P-450. The deduced protein consists of 521 amino acids, contains two putative transmembrane segments in the N-terminal region and has a characteristic heme-binding sequence in the C-terminal part. Sequence alignments with members of 11 reported cytochrome P-450 families revealed a strong homology to an alkane-inducible cytochrome P-450 from Candida tropicalis.
从正构烷烃培养的麦芽糖假丝酵母细胞的聚腺苷酸RNA开始构建cDNA文库，并分离出包含烷烃羟化细胞色素P-450完整编码序列的cDNA克隆。推导的蛋白质由521个氨基酸组成，在N端区域含有两个推定的跨膜区段，并且在C端部分具有特征性的血红素结合序列。与11个已报道的细胞色素P-450家族成员的序列比对显示与来自热带假丝酵母的烷烃诱导型细胞色素P-450具有高度同源性。
