Slomiany B L, Bilski J, Sarosiek J, Murty V L, Dworkin B, VanHorn K, Zielenski J, Slomiany A
Biochem Biophys Res Commun. 1987 Apr 14;144(1):307-14. doi: 10.1016/s0006-291x(87)80511-9.
The role of Campylobacter pyloridis, a spiral bacteria associated with gastritis and peptic ulcers in weakening the mucus component of gastric mucosal barrier was investigated. The colonies of bacteria, cultured from antral mucosal biopsies of patients undergoing gastroscopy, were washed with saline, passed through sterilization filter and the filtrate was examined for protease and glycosylhydrolase activities. The obtained results revealed that the filtrate exhibited a strong proteolytic activity not only towards the typical protein substrates such as albumin but also towards gastric mucin. Optimum enzymatic activity for degradation of mucin was attained at pH 7.0 and the protease activity was found in a low m.w. (less than 50K) protein fraction. The filtrate showed little glycosylhydrolase activity and did not cause the hydrolysis of mucin carbohydrates. The data suggest that C pyloridis infection weakens the gastric mucosal defense by causing proteolytic degradation of mucin component of the protective mucus layer.
幽门弯曲菌是一种与胃炎和消化性溃疡相关的螺旋菌，其在削弱胃黏膜屏障黏液成分方面的作用得到了研究。从接受胃镜检查患者的胃窦黏膜活检组织中培养出的细菌菌落，用盐水洗涤，通过除菌滤器，然后检测滤液中的蛋白酶和糖基水解酶活性。所得结果显示，滤液不仅对典型的蛋白质底物如白蛋白表现出强大的蛋白水解活性，而且对胃黏蛋白也有活性。降解黏蛋白的最佳酶活性在pH 7.0时达到，蛋白酶活性存在于低分子量（小于50K）的蛋白质组分中。滤液显示出很少的糖基水解酶活性，并且不会引起黏蛋白碳水化合物的水解。数据表明，幽门弯曲菌感染通过导致保护性黏液层黏蛋白成分的蛋白水解降解来削弱胃黏膜防御。
