Wang Lin, Zhou Bi-Qiang, Li Ying-Hong, Jiang Qian-Qian, Cong Wei-Hong, Chen Ke-Ji, Wen Xiao-Min, Wu Zheng-Zhi
The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen Institute of Geriatrics, Shenzhen, Guangdong Province, China.
The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, Guangdong Province, China.
Neural Regen Res. 2023 Jan;18(1):226-232. doi: 10.4103/1673-5374.344841.
Previous studies have shown that berberine has neuroprotective effects against Alzheimer's disease, including antagonizing tau phosphorylation, and inhibiting acetylcholinesterase activity and neural cell apoptosis. However, its low bioavailability and adverse reactions with conventional administration limit its clinical application. In this study, we prepared berberine nanoliposomes using liposomes characterized by low toxicity, high entrapment efficiency, and biodegradability, and modified them with lactoferrin. Lactoferrin-modified berberine nanoliposomes had uniform particle size and high entrapment efficiency. We used the lactoferrin-modified berberine nanoliposomes to treat a mouse model of Alzheimer's disease established by injection of amyloid-beta 1-42 into the lateral ventricle. Lactoferrin-modified berberine nanoliposomes inhibited acetylcholinesterase activity and apoptosis in the hippocampus, reduced tau over-phosphorylation in the cerebral cortex, and improved mouse behavior. These findings suggest that modification with lactoferrin can enhance the neuroprotective effects of berberine nanoliposomes in Alzheimer's disease.
先前的研究表明，黄连素对阿尔茨海默病具有神经保护作用，包括拮抗tau蛋白磷酸化、抑制乙酰胆碱酯酶活性和神经细胞凋亡。然而，其低生物利用度以及常规给药时的不良反应限制了其临床应用。在本研究中，我们使用具有低毒性、高包封率和可生物降解性的脂质体制备了黄连素纳米脂质体，并用乳铁蛋白对其进行修饰。乳铁蛋白修饰的黄连素纳米脂质体具有均匀的粒径和高包封率。我们使用乳铁蛋白修饰的黄连素纳米脂质体治疗通过向侧脑室注射淀粉样β蛋白1-42建立的阿尔茨海默病小鼠模型。乳铁蛋白修饰的黄连素纳米脂质体抑制了海马体中的乙酰胆碱酯酶活性和细胞凋亡，减少了大脑皮层中tau蛋白的过度磷酸化，并改善了小鼠行为。这些发现表明，用乳铁蛋白修饰可以增强黄连素纳米脂质体在阿尔茨海默病中的神经保护作用。
