Cai Li-Fei, Wang Shi-Bin, Hou Cai-Lan, Li Ze-Bin, Liao Yi-Jun, Jia Fu-Jun
The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong Province, People's Republic of China.
Guangdong Mental Health Center, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong Province, People's Republic of China.
Neuropsychiatr Dis Treat. 2022 Jul 1;18:1315-1328. doi: 10.2147/NDT.S360588. eCollection 2022.
By exploring the gut-related microbiota differences of adolescents with non-suicidal self-injury (NSSI) and depression (without NSSI) and healthy volunteers, we provide a theoretical basis for the prevention and control of NSSI in adolescents through intestinal microecological regulation.
A total of 99 subjects were recruited in Guangdong Province, China, including 51 adolescents with NSSI (KD), 24 healthy adolescents (NOR1), and 24 depression adolescents without NSSI (NOR2). General clinical data and fecal samples were collected from all subjects, who were assessed using the NSSI Behavioral Questionnaire and the 24-item Hamilton Depression Scale. The taxonomic composition of the gut microbiota was determined using the 16S rDNA gene sequencing method.
There were significant differences in diversity between the KD and NOR1, and the species uniformity index of the KD according to the Shannon and Simpson indices was significantly reduced compared with that of the NOR1 (4.81 vs 5.21, p<0.01; 0.02 vs 0.01, p<0.05). The relative abundances were different among the KD, NOR1 and NOR2, as reflected at the taxonomic levels of class, order, family, genus, and species. Bacteroides were the dominant flora of the KD and NOR2, while Mitsuokella was the dominant flora that distinguished the KD from the NOR2.
We found that gut microbiota diversity was decreased in adolescents with NSSI, and the relative abundance was altered at different taxonomic levels. These results enrich the understanding of the relationship between NSSI and depression and the gut microbiota, Supporting that NSSI and depression are not homologous disorders. What is more, it establishes the basis for exploring the mechanisms of flora action in NSSI, providing a possible direction for NSSI to achieve a better prognosis and prevent relapse.
通过探究非自杀性自伤（NSSI）青少年、抑郁症（无NSSI）青少年与健康志愿者肠道相关微生物群的差异，为通过肠道微生态调节预防和控制青少年NSSI提供理论依据。
在中国广东省共招募了99名受试者，包括51名有NSSI的青少年（KD组）、24名健康青少年（NOR1组）和24名无NSSI的抑郁症青少年（NOR2组）。收集所有受试者的一般临床资料和粪便样本，使用NSSI行为问卷和24项汉密尔顿抑郁量表进行评估。采用16S rDNA基因测序方法确定肠道微生物群的分类组成。
KD组与NOR1组之间的多样性存在显著差异，根据香农指数和辛普森指数，KD组的物种均匀度指数与NOR1组相比显著降低（4.81对5.21，p<0.01；0.02对0.01，p<0.05）。KD组、NOR1组和NOR2组之间的相对丰度不同，在纲、目、科、属和种的分类水平上均有体现。拟杆菌是KD组和NOR2组的优势菌群，而 Mitsuokella 是区分KD组与NOR2组的优势菌群。
我们发现有NSSI的青少年肠道微生物群多样性降低，且在不同分类水平上相对丰度发生改变。这些结果丰富了对NSSI与抑郁症及肠道微生物群之间关系的认识，支持NSSI和抑郁症并非同源性疾病。此外，它为探索NSSI中菌群作用机制奠定了基础，为NSSI实现更好的预后和预防复发提供了可能的方向。
