Mohammed Asmaa, Shaker Olfat G, Khalil Mahmoud A F, Elsabagh Yumn A, Gomaa Mohammed, Ahmed Azza M, Erfan Randa
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Saudi J Biol Sci. 2022 Aug;29(8):103344. doi: 10.1016/j.sjbs.2022.103344. Epub 2022 Jun 16.
Behçet's disease (BD) is a chronic inflammatory disease. Immunological defects have been shown to play a significant role in the progression of BD. The serum levels of two long non-coding RNAs (lncRNAs), NEAT1 and MALAT1, were examined in patients with BD to identify their role in the disease pathogenesis. Both lncRNAs were mentioned as essential regulators of innate immune responses and have a crucial role in inflammatory diseases. Fifty patients with BD and a similar number of control individuals were involved in our study. At enrollment, data was collected from patients and controls, and the disease severity in active cases was determined using the Behçet's Disease Current Activity Form (BDCAF). Levels of the two studied biomarkers in the serum, NEAT1 and MALAT1, were investigated by quantitative RT-PCR (qRT-PCR). NEAT1 levels were significantly turned down in BD patients (fold changes = 0.77, ) and correlated negatively with the BDCAF (r = -0.41; = 0.003). On the other hand, the MALAT1 levels were significantly up-regulated in BD patients (fold changes = 2.65, = 0.003). Serum levels of NEAT1 were significantly decreased in patients with active states than in stationary cases (0.387 versus 1.99, respectively; ) and compared with controls (). Also, NEAT1 levels were significantly increased in patients with stationary states compared to controls (). There was a positive association between NEAT1 and MALAT1 levels among BD patients (r = 0.29, Our findings demonstrate a possible role of NEAT1 and MALAT1 in the pathogenesis of BD.
白塞病（BD）是一种慢性炎症性疾病。免疫缺陷已被证明在BD的进展中起重要作用。检测了BD患者血清中两种长链非编码RNA（lncRNA），即NEAT1和MALAT1的水平，以确定它们在疾病发病机制中的作用。这两种lncRNA均被认为是先天免疫反应的重要调节因子，在炎症性疾病中起关键作用。我们的研究纳入了50例BD患者和数量相近的对照个体。在入组时，收集了患者和对照的数据，并使用白塞病当前活动表（BDCAF）确定活动期病例的疾病严重程度。通过定量逆转录聚合酶链反应（qRT-PCR）检测血清中两种研究生物标志物NEAT1和MALAT1的水平。BD患者的NEAT1水平显著降低（倍数变化=0.77，），且与BDCAF呈负相关（r=-0.41；=0.003）。另一方面，BD患者的MALAT1水平显著上调（倍数变化=2.65，=0.003）。活动期患者的血清NEAT1水平显著低于静止期患者（分别为0.387和1.99；），且与对照组相比也较低（）。与对照组相比，静止期患者的NEAT1水平也显著升高（）。BD患者中NEAT1和MALAT1水平之间存在正相关（r=0.29，我们的研究结果表明NEAT1和MALAT1在BD发病机制中可能发挥作用。
