Jolayemi Akinyemi K, Adeyemi David O, Awoniran Paul O
Department of Human Anatomy, Faculty of Basic Medical Sciences, Redeemer's University, Ede, Osun State, Nigeria.
Department of Anatomy and Cell Biology, Faculty of Basic Medical Sciences, College of Health Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
Vet Anim Sci. 2022 Jun 26;17:100260. doi: 10.1016/j.vas.2022.100260. eCollection 2022 Sep.
In this study, the effect of orally administered methanolic extract of stem bark (MLSB) was tested for its efficacy to reverse lead nitrate-induced hepatotoxicity in Wistar rats. Thirty-six female rats were assigned into six groups (n = 6). Rats in group I received 2.2 mL/kg distilled water for 28 days, those in group II received 30 mg/kg lead nitrate for 14 days while those in groups III to VI received 30 mg/kg lead nitrate for 14 days followed by a treatment with 100, 250, 500 mg/kg BW MLSB extract and 0.2 mL/100 kg rats silymarin respectively for 14 days. They were sacrificed after 28 days after which biochemical, histological, and immunohistochemical parameters were examined. The results of this study showed a reduction of catalase and superoxide dismutase activities by lead nitrate. Deranged hepatic histomorphology was also observed intracellularly and extracellularly in lead nitrate-treated rats. Altered vimentin arrangement was also observed in lead nitrate-treated rats. However, 250 mg/kg BW dose of significantly reversed some of these changes while the 500 mg had some toxic effect on liver tissue. We concluded that the extract at 250mg/kg BW dose may be a potential treatment for conditions associated with lead toxicity and other metallic particles.
在本研究中，测试了口服茎皮甲醇提取物（MLSB）对逆转硝酸铅诱导的Wistar大鼠肝毒性的功效。将36只雌性大鼠分为六组（n = 6）。第一组大鼠接受2.2 mL/kg蒸馏水，持续28天；第二组大鼠接受30 mg/kg硝酸铅，持续14天；而第三组至第六组大鼠接受30 mg/kg硝酸铅，持续14天，随后分别用100、250、500 mg/kg体重的MLSB提取物和0.2 mL/100 kg大鼠水飞蓟宾处理14天。28天后将它们处死，然后检查生化、组织学和免疫组化参数。本研究结果表明，硝酸铅会降低过氧化氢酶和超氧化物歧化酶的活性。在硝酸铅处理的大鼠中，细胞内和细胞外还观察到肝脏组织形态学紊乱。在硝酸铅处理的大鼠中还观察到波形蛋白排列改变。然而，250 mg/kg体重剂量的提取物显著逆转了其中一些变化，而500 mg/kg体重剂量对肝脏组织有一定毒性作用。我们得出结论，250mg/kg体重剂量的提取物可能是治疗与铅毒性和其他金属颗粒相关病症的潜在药物。
