Loop-mediated isothermal amplification (LAMP) for rapid and easy identification of .

is a major toxic mushroom in Japan. When food poisoning caused by occurs, quick and accurate identification using a method that does not rely on morphological discrimination is required. Because the loop-mediated isothermal amplification (LAMP) method meets these requirements, we developed a LAMP method for detecting . Amplification occurred within 60 min, and the presence or absence of was confirmed within 2 h, including the DNA extraction protocol. The LAMP method did not show cross-reactivity with 13 species of edible mushrooms, had high specificity toward , and had sufficient detection sensitivity even in a mixed mushroom sample containing 1% . Additionally, could be detected in simulated food poisoning samples of heated and digested mushrooms, and in actual food poisoning residual samples.