Rogers Ariel T, Bullard Kaitlin R, Dod Akash C, Wang Yong
Department of Physics, University of Arkansas, Fayetteville, AR, USA.
Materials Science and Engineering Program, University of Arkansas, Fayetteville, AR, USA.
Bio Protoc. 2022 May 5;12(9):e4410. doi: 10.21769/BioProtoc.4410.
Bacterial studies based on growth curves are common in microbiology and related fields. Compared to the standard photometer and cuvette based protocols, bacterial growth curve measurements with microplate readers provide better temporal resolution, higher efficiency, and are less laborious, while analysis and interpretation of the microplate-based measurements are less straightforward. Recently, we developed a new analysis method for evaluating bacterial growth with microplate readers based on time derivatives. Here, we describe a detailed protocol for this development and provide the homemade program for the new analysis method.
基于生长曲线的细菌研究在微生物学及相关领域很常见。与基于标准光度计和比色皿的实验方案相比，使用酶标仪进行细菌生长曲线测量具有更好的时间分辨率、更高的效率且更省力，而基于酶标仪测量的分析和解释则没那么直接。最近，我们开发了一种基于时间导数用酶标仪评估细菌生长的新分析方法。在此，我们描述了该方法开发的详细方案，并提供了新分析方法的自制程序。
