Mikel U V
Anal Quant Cytol Histol. 1987 Mar;9(1):13-6.
Quantitative electron microscopy (QEM) and microspectrophotometry were used to correlate the Feulgen stain absorption values to the calculated picograms of DNA. Measurements were made in human lymphocytes, rainbow trout lymphocytes and nuclei of trout erythrocytes. The median dry weight of the nucleus, as determined by QEM, was 35.9 pg for a human lymphocyte and 30.5 pg for a trout lymphocyte. Using Salzman's value of 20% DNA per chromosome (i.e., chromatin), a human lymphocyte nucleus thus contains 7.18 pg of DNA and a trout lymphocyte nucleus 6.1 pg of DNA. The mean Feulgen absorption value of the nucleus, given in arbitrary units (AU), was 14.5 for a human lymphocyte, 12.7 for a trout lymphocyte and 12.0 for a trout erythrocyte. From these values, it was derived that each picogram of DNA of a human lymphocyte nucleus is represented by 2.02 arbitrary Feulgen units while the values for trout nuclei were 2.08 AU and 1.97 AU. On the average, we find that each picogram of DNA is represented by two arbitrary Feulgen units in our microspectrophotometric measurements.
采用定量电子显微镜（QEM）和显微分光光度法，将福尔根染色吸收值与计算得出的DNA皮克数进行关联。对人类淋巴细胞、虹鳟淋巴细胞和虹鳟红细胞核进行了测量。通过QEM测定，人类淋巴细胞细胞核的干重中位数为35.9皮克，虹鳟淋巴细胞细胞核的干重中位数为30.5皮克。根据萨尔兹曼提出的每条染色体（即染色质）含20%DNA的数值，一个人类淋巴细胞细胞核因此含有7.18皮克DNA，一个虹鳟淋巴细胞细胞核含有6.1皮克DNA。细胞核的福尔根平均吸收值以任意单位（AU）表示，人类淋巴细胞为14.5，虹鳟淋巴细胞为12.7，虹鳟红细胞为12.0。从这些数值可以得出，人类淋巴细胞细胞核每皮克DNA由2.02个任意福尔根单位表示，而虹鳟细胞核的数值分别为2.08 AU和1.97 AU。在我们的显微分光光度测量中，平均而言，每皮克DNA由两个任意福尔根单位表示。
