Rosenthal D L, Manjikian V
Anal Quant Cytol Histol. 1987 Mar;9(1):55-9.
The computer-assisted microscope demands rigid specifications for specimen preparation. This paper addresses the variety of techniques developed by researchers attempting to automatically screen uterine cervical specimens. These same techniques could also be utilized for specimens from other body sites. In contrast to the easily prepared routine Papanicolaou smear, these techniques can be broken down into various steps as follows: transport media, cellular disaggregation, cell number estimation, cell separation and specimen enrichment, cellular adhesion to glass slide and cell transfer onto the slide. Variations on the theme are contrasted among specimen preparation methods utilized by prominent research groups. The plea for a simpler preparation method or, hopefully, utilization of the routine Papanicolaou smear for computer-assisted microscopy is made.
计算机辅助显微镜对标本制备有严格的规格要求。本文探讨了研究人员为自动筛查子宫颈标本而开发的多种技术。这些相同的技术也可用于来自身体其他部位的标本。与易于制备的常规巴氏涂片不同，这些技术可细分为以下各个步骤：运输介质、细胞解离、细胞数量估计、细胞分离和标本富集、细胞黏附于载玻片以及细胞转移到载玻片上。主要研究小组使用的标本制备方法之间存在对该主题的不同变体。文中呼吁采用更简单的制备方法，或者希望能将常规巴氏涂片用于计算机辅助显微镜检查。
