College of Horticulture, Northwest Agricultural and Forestry University, Yangling 712100, China.
Plant Dis. 2023 Feb;107(2):306-314. doi: 10.1094/PDIS-03-22-0607-RE. Epub 2023 Feb 16.
Environmental temperature and humidity play a vital role in plant-pathogen interactions, which profoundly affect the occurrence of crop diseases. However, the specific methods and mechanisms through which intermittent changes in temperature and humidity mitigate plant diseases remain unclear. In this study, six temperature and humidity combinations were set, the disease severity of tomatoes and biomass of were analyzed, and the infection process of pathogens was observed using an optical microscope. Furthermore, dual RNA-seq analysis was performed to explore the interactions between plants and pathogens. Results showed that the 24 hours postinoculation (hpi)-12 h day (regulation was performed at 24 hpi for 12 h after inoculation during the day) treatment reduced the gray mold severity and biomass of in plants by the greatest amount and effectively inhibited the growth of mycelia. The 24 hpi-12 h day treatment induced the upregulation of light reactions, photorespiration, and Calvin cycle-related genes in tomatoes, whereas fungal genes related to the biosynthesis of sesquiterpene botrydial and polyketide botcinic acid were downregulated. Overall, we identified the optimal combination of temperature and humidity changes to inhibit the development of tomato gray mold and preliminarily explored the interactions between tomato and under temperature and humidity changes. This work has practical importance and provides a theoretical basis for the ecological control of plant diseases.
环境温度和湿度在植物-病原菌相互作用中起着至关重要的作用，它们深刻影响着作物病害的发生。然而，温度和湿度间歇性变化减轻植物病害的具体方法和机制尚不清楚。本研究设置了 6 种温度和湿度组合，分析了番茄的病情严重度和生物量，并使用光学显微镜观察病原菌的感染过程。此外，还进行了双 RNA-seq 分析，以探讨植物与病原菌之间的相互作用。结果表明，24 小时接种后 12 小时白天（24 小时接种后白天进行 12 小时处理）处理可最大程度地降低番茄灰霉病的严重度和生物量，并有效抑制菌丝生长。24 小时接种后 12 小时白天处理诱导番茄中光反应、光呼吸和卡尔文循环相关基因的上调，而真菌中与倍半萜 botrydial 和聚酮 botcinic 酸生物合成相关的基因则下调。总之，我们确定了抑制番茄灰霉病发展的最佳温度和湿度变化组合，并初步探讨了温度和湿度变化下番茄与灰葡萄孢菌之间的相互作用。这项工作具有实际意义，为植物病害的生态防治提供了理论依据。
