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通过深层发酵将菠萝蜜种子废料生物转化为真菌生物质蛋白

Bioconversion of Jackfruit Seed Waste to Fungal Biomass Protein by Submerged Fermentation.

作者信息

Chakraborty Ankita, Bhowal Jayati

机构信息

School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, Howrah, 711 103, West Bengal, India.

出版信息

Appl Biochem Biotechnol. 2023 Apr;195(4):2158-2171. doi: 10.1007/s12010-022-04063-8. Epub 2022 Jul 8.

Abstract

The bioconversion of jackfruit seed into a valuable product like Single-Cell Protein (SCP) was carried out using Generally Recognized As Safe (GRAS), five filamentous fungi such as Penicillium expansum, Aspergillus niger, Aspergillus oryzae, Rhizopus microsporus, and Candida intermedia by submerged fermentation. Data obtained from the study showed that supplementation of jackfruit seed extracts with inorganic nitrogen sources and glucose as a carbon source enhanced fungal biomass and SCP production. Among the various fungal strains, A. niger gave the highest biomass and protein yield of 4.01 ± 0.07 g/l and 1.82 ± 0.03 g/l, respectively, on the 9th day of fermentation followed by P. expansum (3.65 ± 0.04 g/l and 1.68 ± 0.03 g/l, respectively). C. intermedia growth was not recorded in all the experimental media. The present study revealed that fungal biomass has shown low crude fat, crude fiber, and total genomic DNA content ranged from 1.10 ± 0.20 to 4.95 ± 0.40%. A. niger and P. expansum were the most efficient in the conversion of sugar (55.83 ± 0.3% and 54.71 ± 0.4%, respectively) to yield biomass in sugar supplementation media. P. expansum and A. niger were the most promising fungal strains to produce fungal biomass protein using inexpensive agro-waste materials.

摘要

利用被普遍认为安全(GRAS)的5种丝状真菌,即扩展青霉、黑曲霉、米曲霉、微小根毛霉和间型假丝酵母,通过深层发酵将菠萝蜜种子生物转化为单细胞蛋白(SCP)这种有价值的产品。该研究获得的数据表明,用无机氮源和葡萄糖作为碳源补充菠萝蜜种子提取物可提高真菌生物量和SCP产量。在各种真菌菌株中,黑曲霉在发酵第9天的生物量和蛋白质产量最高,分别为4.01±0.07 g/l和1.82±0.03 g/l,其次是扩展青霉(分别为3.65±0.04 g/l和1.68±0.03 g/l)。在所有实验培养基中均未记录到间型假丝酵母的生长。本研究表明,真菌生物量的粗脂肪、粗纤维含量较低,总基因组DNA含量在1.10±0.20%至4.95±0.40%之间。在添加糖的培养基中,黑曲霉和扩展青霉在将糖转化为生物量方面效率最高(分别为55.83±0.3%和54.71±0.4%)。扩展青霉和黑曲霉是利用廉价农业废料生产真菌生物量蛋白最有前景的真菌菌株。

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