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丁香假单胞菌番茄致病变种对罗勒和迷迭香次生代谢产物提取物的体外抑制作用。

In Vitro Inhibitory Effect of Some Secondary Metabolites Extract from Ocimum basilicum and Salvia officinalis Against Fusarium oxysporum f.sp albedinis of Phoenix dactylifera. L.

机构信息

Department of Biology, Faculty of Sciences of Nature and Life, Biology Department, University Tahri Mohammed, BP 417, Bechar, Algeria.

Laboratory of Plant Resource Development and Food Security in Semi Arid Areas, South West of Algeria, Department of Biology, University of Bechar, Bechar, Algeria.

出版信息

Appl Biochem Biotechnol. 2022 Dec;194(12):5655-5665. doi: 10.1007/s12010-022-04067-4. Epub 2022 Jul 8.

DOI:10.1007/s12010-022-04067-4
PMID:35802242
Abstract

Fusarium of date palm is a vascular fusariosis caused by a telluric fungus Fusarium oxysporum f.sp. albedinis (F.O.A) which affects the best date varieties in Algeria. Several medicinal plants are adapted with the climatic complexity of the Saharan region, among these plants in the south-west of Algeria Salvia officinalis and Ocimum basilicum. The aim of this work is to study the antifungal effect of flavonoid extracts from Salvia officinalis and Ocimum basilicum leaves by the technique described by Bouchelta et al. (2005), as well the antifungal effect of the aqueous extract of these plants against Fusarium oxysporum f.sp. albedinis isolated from the grove of Igli. The evaluation of the antifungal activity revealed a slight mycelial growth anti-proportional to the concentration of flavonoid extract added to the culture medium with an inhibition index of 73.04% at the concentration 3.17 μg/ml and 57.42% at the concentration 1.13 µg/ml for flavonoids extract of Ocimum basilicum and Salvia officinalis, respectively. In addition, the aqueous extract revealed an inhibition index of 35.15% at the concentration of 0.33 mg/ml and 10.54% at the concentration of 3.92 mg/ml for Salvia officinalis and Ocimum basilicum, respectively.

摘要

棕榈镰刀菌是一种血管镰刀菌病,由土生真菌尖孢镰刀菌复合种(F.O.A)引起,影响阿尔及利亚最好的枣品种。几种药用植物适应了撒哈拉地区的气候复杂性,其中包括阿尔及利亚西南部的鼠尾草和罗勒。这项工作的目的是研究 Salvia officinalis 和 Ocimum basilicum 叶中类黄酮提取物的抗真菌作用,方法是采用 Bouchelta 等人(2005 年)描述的技术,以及这些植物的水提物对从 Igli 树林中分离出的尖孢镰刀菌复合种的抗真菌作用。抗真菌活性的评估显示,与添加到培养基中的类黄酮提取物浓度成比例的菌丝生长略有抑制,其中 Ocimum basilicum 和 Salvia officinalis 的类黄酮提取物的抑制指数分别为 73.04%(浓度为 3.17μg/ml)和 57.42%(浓度为 1.13μg/ml)。此外,水提物对 Salvia officinalis 和 Ocimum basilicum 的抑制指数分别为 35.15%(浓度为 0.33mg/ml)和 10.54%(浓度为 3.92mg/ml)。

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