Department of Chemistry, Purdue University, West Lafayette, IN-47907, USA.
Chembiochem. 2022 Sep 16;23(18):e202200306. doi: 10.1002/cbic.202200306. Epub 2022 Jul 27.
Tile-based DNA self-assembly is a powerful approach for nano-constructions. In this approach, individual DNA single strands first assemble into well-defined structural tiles, which, then, further associate with each other into final nanostructures. It is a general assumption that the lower-level structures (tiles) determine the higher-level, final structures. In this study, we present concrete experimental data to show that higher-level structures could, at least in the current example, also impact on the formation of lower-level structures. This study prompts questions such as: how general is this phenomenon in programmed DNA self-assembly and can we turn it into a useful tool for fine tuning DNA self-assembly?
基于瓦片的 DNA 自组装是一种强大的纳米结构构建方法。在这种方法中，单个 DNA 单链首先组装成具有明确定义结构的瓦片，然后这些瓦片进一步相互关联形成最终的纳米结构。人们普遍认为，较低层次的结构（瓦片）决定了较高层次的最终结构。在这项研究中，我们提供了具体的实验数据，表明较高层次的结构至少在当前的例子中也会影响较低层次结构的形成。这项研究引发了一些问题，例如：这种现象在可编程 DNA 自组装中普遍存在吗？我们能否将其转化为精细调整 DNA 自组装的有用工具？
