Tandon Anshula, Song Yongwoo, Mitta Sekhar Babu, Yoo Sanghyun, Park Suyoun, Lee Sungjin, Raza Muhammad Tayyab, Ha Tai Hwan, Park Sung Ha
Department of Physics and Sungkyunkwan Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon 16419, Korea.
Future Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea.
ACS Nano. 2020 May 26;14(5):5260-5267. doi: 10.1021/acsnano.0c01387. Epub 2020 Mar 16.
Owing to its high information density, energy efficiency, and massive parallelism, DNA computing has undergone several advances and made significant contributions to nanotechnology. Notably, arithmetic calculations implemented by multiple logic gates such as adders and subtractors have received much attention because of their well-established logic algorithms and feasibility of experimental implementation. Although small molecules have been used to implement these computations, a DNA tile-based calculator has been rarely addressed owing to complexity of rule design and experimental challenges for direct verification. Here, we construct a DNA-based calculator with three types of building blocks (propagator, connector, and solution tiles) to perform addition and subtraction operations through algorithmic self-assembly. An atomic force microscope is used to verify the solutions. Our method provides a potential platform for the construction of various types of DNA algorithmic crystals (such as flip-flops, encoders, and multiplexers) by embedding multiple logic gate operations in the DNA base sequences.
由于其高信息密度、能源效率和大规模并行性，DNA计算已取得多项进展，并为纳米技术做出了重大贡献。值得注意的是，由加法器和减法器等多个逻辑门实现的算术计算因其成熟的逻辑算法和实验实现的可行性而备受关注。尽管小分子已被用于实现这些计算，但由于规则设计的复杂性和直接验证的实验挑战，基于DNA瓦片的计算器很少被提及。在这里，我们构建了一个基于DNA的计算器，它由三种类型的构建块（传播器、连接器和溶液瓦片）组成，通过算法自组装来执行加法和减法运算。使用原子力显微镜来验证解决方案。我们的方法通过将多个逻辑门操作嵌入DNA碱基序列中，为构建各种类型的DNA算法晶体（如触发器、编码器和多路复用器）提供了一个潜在的平台。
