Zheng Mengxi, Li Zhe, Liu Longfei, Li Mo, Paluzzi Victoria E, Hyun Choi Jong, Mao Chengde
J Am Chem Soc. 2021 Dec 8;143(48):20363-20367. doi: 10.1021/jacs.1c09925. Epub 2021 Nov 22.
DNA origami is a powerful method for constructing DNA nanostructures. It requires long single-stranded DNAs. The preparation of such long DNA strands is often quite tedious and has a limited production yield. In contrast, duplex DNAs can be easily prepared via enzymatic reactions in large quantities. Thus, we ask a question: can we design DNA nanostructures in such a way that the two complementary strands can simultaneously fold into the designed structures in the same solution instead of hybridizing with each other to form a DNA duplex? By engineering DNA interaction kinetics, herein we are able to provide multiple examples to concretely demonstrate a positive answer to this question. The resulting DNA nanostructures have been thoroughly characterized by electrophoresis and atomic force microscopy imaging. The reported strategy is compatible with the DNA cloning method and thus would provide a convenient method for the large-scale production of the designed DNA nanostructures.
DNA折纸术是构建DNA纳米结构的一种强大方法。它需要长单链DNA。制备如此长的DNA链通常相当繁琐，且产量有限。相比之下，双链DNA可以通过酶促反应轻松大量制备。因此，我们提出一个问题：我们能否以这样一种方式设计DNA纳米结构，即两条互补链能够在同一溶液中同时折叠成设计的结构，而不是相互杂交形成DNA双链？通过设计DNA相互作用动力学，我们在此能够提供多个实例来具体证明对这个问题的肯定答案。所得的DNA纳米结构已通过电泳和原子力显微镜成像进行了全面表征。所报道的策略与DNA克隆方法兼容，因此将为大规模生产设计的DNA纳米结构提供一种便捷方法。
