Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry & Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei Key Laboratory of Ferro & Piezoelectric Materials and Devices, College of Chemistry & Chemical Engineering, Hubei University, Wuhan, 430062, PR China; Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, National Reference Lab for Biotoxin Test, Hubei Hongshan Laboratory, Wuhan, 430062, PR China.
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, National Reference Lab for Biotoxin Test, Hubei Hongshan Laboratory, Wuhan, 430062, PR China.
Biosens Bioelectron. 2022 Oct 15;214:114523. doi: 10.1016/j.bios.2022.114523. Epub 2022 Jun 30.
Designing a photoelectrochemical (PEC) biosensor with preponderant sensitivity and anti-interference is a challenge for detecting small molecules in real samples with complex matrices. To this end, the BiO/CuBiO was synthesized in one step to enhance visible light's absorption ability, transferring the interfacial carrier's efficiency, a high-active Z-scheme heterojunction, and a photocathode biosensor was proposed. For the first time, we used the density functional theory to verify a Z-scheme transfer pathway of photogenerated electrons in BiO/CuBiO and the energy band structure of BiO and CuBiO, respectively. BiO/CuBiO-based PEC biosensor was developed for competive immunoassay of small molecular, aflatoxin B1 (AFB1) as an example, resulting in a low detection limit of 297.4 fg/mL and a linear range of 1.4 pg/mL-280 ng/mL in urine, water, peanut, and wheat samples. Using spiked experiments, the satisfied repeatability, reproducibility, stability, and specificity of the BiO/CuBiO-based PEC biosensor indicated a promise for application in health care, the environment, and food.
设计具有优越灵敏度和抗干扰能力的光电化学（PEC）生物传感器，是在具有复杂基质的实际样品中检测小分子的挑战。为此，我们一步合成了 BiO/CuBiO 以增强可见光的吸收能力，提高界面载流子的效率，构建了高效的 Z 型异质结，并提出了一种光阴极生物传感器。首次使用密度泛函理论分别验证了 BiO/CuBiO 中光生电子的 Z 型转移途径和 BiO 和 CuBiO 的能带结构。我们基于 BiO/CuBiO 开发了用于小分子竞争免疫分析的 PEC 生物传感器，以黄曲霉毒素 B1（AFB1）为例，在尿液、水、花生和小麦样品中检测限低至 297.4 fg/mL，线性范围为 1.4 pg/mL-280 ng/mL。通过加标实验，BiO/CuBiO 基 PEC 生物传感器具有良好的重复性、重现性、稳定性和特异性，有望在医疗保健、环境和食品领域得到应用。
