Chen Di-Nan, Wang Gui-Qing, Mei Li-Ping, Feng Jiu-Ju, Wang Ai-Jun
Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China.
Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, China.
Biosens Bioelectron. 2023 Mar 1;223:115038. doi: 10.1016/j.bios.2022.115038. Epub 2022 Dec 27.
As one of the most toxic chemical substances, aflatoxin B1 (AFB1) has a strong carcinogenic effect even at a trace level in human and animal, which severely threatens human health and even causes cancers. Therefore, ultrasensitive detection of AFB1 is of significant importance. For such analysis, dual II-scheme sheet-like BiS/BiO/AgS heterostructures were prepared by the in-situ growth method, which exhibited high separation efficiency for the electron-hole (e-h) pairs, prominent stability, and high photoactivity. Moreover, the dendritic nanorod-like Au@Pd@Pt (Au@Pd@Pt DNRs) nanozyme was homely synthesized, whose peroxidase-like activity was scrupulously investigated by catalytical oxidation of diaminobenzidine (DAB) in the presence of HO. Integration by the aptasensing strategy, a photoelectrochemical (PEC) "signal-on" aptasensor was prepared, which exhibited a broader linear range of 0.5 pg mL-100 ng mL with a lower limit of detection (LOD = 0.09 pg mL, S/N = 3). This work provides a feasible strategy to develop advanced PEC biosensors for actual analysis of environmental pollutants.
作为毒性最强的化学物质之一，黄曲霉毒素B1（AFB1）即使在痕量水平下对人和动物也具有很强的致癌作用，严重威胁人类健康甚至引发癌症。因此，对AFB1进行超灵敏检测具有重要意义。针对此类分析，通过原位生长法制备了双II型片状BiS/BiO/AgS异质结构，其对电子-空穴（e-h）对具有高分离效率、出色的稳定性和高光活性。此外，还简便合成了树枝状纳米棒状Au@Pd@Pt（Au@Pd@Pt DNRs）纳米酶，并在HO存在的情况下通过催化氧化二氨基联苯胺（DAB）对其类过氧化物酶活性进行了严格研究。通过适体传感策略进行整合，制备了一种光电化学（PEC）“信号开启”适体传感器，其线性范围更宽，为0.5 pg mL-100 ng mL，检测下限（LOD = 0.09 pg mL，S/N = 3）。这项工作为开发用于实际分析环境污染物的先进PEC生物传感器提供了一种可行的策略。
